Detailed information of TA system TA109268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2262368..2262957
• Replicon: chromosome
• Accession: NZ_CP031500
• Organism: Deinococcus radiodurans strain R1 dM1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q9RX98
• Locus tag: DXG80_RS11720
• Protein ID: WP_010887062.1
• Coordinates: 2262604..2262957 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q9RX99
• Locus tag: DXG80_RS11715
• Protein ID: WP_010887061.1
• Coordinates: 2262368..2262610 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXG80_RS11680	2257380..2257799	+	420	WP_010887054.1	DinB family protein	-
DXG80_RS11685	2257792..2258163	+	372	Protein_2210	inorganic pyrophosphatase	-
DXG80_RS11690	2258160..2258843	+	684	WP_010887056.1	SDR family oxidoreductase	-
DXG80_RS11695	2258864..2259826	+	963	WP_034349507.1	NAD(P)/FAD-dependent oxidoreductase	-
DXG80_RS11700	2259849..2260028	+	180	WP_034349506.1	hypothetical protein	-
DXG80_RS11705	2260030..2260659	+	630	WP_027479568.1	HAD family hydrolase	-
DXG80_RS11710	2260707..2262188	-	1482	Protein_2215	carboxylesterase/lipase family protein	-
DXG80_RS11715	2262368..2262610	+	243	WP_010887061.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DXG80_RS11720	2262604..2262957	+	354	WP_010887062.1	endoribonuclease MazF	Toxin
DXG80_RS11725	2263010..2263234	+	225	WP_162177573.1	hypothetical protein	-
DXG80_RS11730	2263231..2263644	+	414	WP_010887064.1	type II toxin-antitoxin system VapC family toxin	-
DXG80_RS11735	2263641..2266109	+	2469	WP_010887065.1	ATP-dependent helicase HrpB	-
DXG80_RS11740	2266290..2266514	-	225	WP_162177572.1	hypothetical protein	-
DXG80_RS11745	2266670..2267446	-	777	WP_010887067.1	trans-aconitate 2-methyltransferase	-
DXG80_RS11750	2267567..2267746	-	180	WP_028328008.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-112)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12450.24 Da
• Isoelectric Point: 7.3219

>T109268 WP_010887062.1 NZ_CP031500:2262604-2262957 [Deinococcus radiodurans]
MVSDYVPDAGHLVWLNFTPQAGHEQGGRRPALVLSPAAYNGVTGLMQACPVTSRAKGYPFEVTLPAHLGVSGVVLADHCR
SLDWRSRRAEQLAEAPADVLAEVRGKLGSLLGMSEKA

Nucleotide

• Length: 354 bp

>T109268 NZ_CP031500:2262604-2262957 [Deinococcus radiodurans]
ATGGTAAGCGATTATGTCCCGGACGCTGGGCATCTGGTGTGGCTCAACTTCACACCTCAGGCAGGGCATGAACAGGGGGG
ACGGCGACCGGCGCTGGTGCTTTCGCCTGCCGCTTATAACGGCGTCACTGGACTGATGCAGGCGTGTCCGGTGACCAGCC
GCGCCAAGGGGTATCCGTTTGAAGTGACGCTCCCTGCTCACCTTGGAGTCAGCGGCGTGGTGCTGGCCGACCATTGCCGC
AGCCTCGACTGGCGCAGTCGCCGGGCCGAACAACTTGCGGAGGCGCCGGCGGACGTCCTGGCCGAAGTCCGTGGCAAGTT
GGGCAGCTTGCTGGGCATGTCCGAGAAGGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8911.12 Da
• Isoelectric Point: 4.3178

>AT109268 WP_010887061.1 NZ_CP031500:2262368-2262610 [Deinococcus radiodurans]
MTSQIQKWGNSLALRIPKALAQQVGLTQSSEVELLLQDGQIVIRPVPARQYDLAALLAEMTPENLHGETDWGALEGREEW

Nucleotide

• Length: 243 bp

>AT109268 NZ_CP031500:2262368-2262610 [Deinococcus radiodurans]
ATGACGAGTCAAATTCAGAAATGGGGCAACAGCCTCGCGCTCCGCATTCCCAAAGCTCTGGCGCAGCAGGTGGGACTGAC
GCAGAGTTCAGAAGTGGAGCTGCTTCTTCAGGACGGTCAGATTGTCATCCGGCCAGTTCCTGCTCGGCAGTACGATCTCG
CCGCGCTGCTGGCCGAAATGACACCTGAAAATCTGCATGGGGAAACAGACTGGGGCGCACTGGAAGGACGCGAGGAATGG
TAA

Structures

Toxin

• PDB: 7D2M
• PDB: 7D2P
• PDB: 7DHP
• PDB: 7D2Q
• PDB: 7D28
• PDB: 7D2N

Antitoxin

• PDB: 7D2P
• PDB: 7D2Q
• PDB: 7D2N
• AlphaFold DB: Q9RX99