Detailed information of TA system TA109222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 2844814..2845370
• Replicon: chromosome
• Accession: NZ_CP031456
• Organism: Xanthomonas oryzae pv. oryzae strain HuN37

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: BRM60_RS14170
• Protein ID: WP_027703932.1
• Coordinates: 2844814..2845104 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A854CIX2
• Locus tag: BRM60_RS14175
• Protein ID: WP_011408759.1
• Coordinates: 2845092..2845370 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BRM60_RS14120	2840584..2841675	+	1092	WP_041182166.1	ribonuclease D	-
BRM60_RS14125	2841751..2842088	-	338	Protein_2545	ATP-dependent DNA ligase	-
BRM60_RS24555	2842148..2842354	-	207	Protein_2546	hypothetical protein	-
BRM60_RS24845	2842370..2842546	-	177	Protein_2547	DUF3606 domain-containing protein	-
BRM60_RS14140	2842768..2843169	+	402	WP_011259345.1	H-NS histone family protein	-
BRM60_RS14160	2844033..2844212	-	180	WP_015463309.1	hypothetical protein	-
BRM60_RS24850	2844483..2844737	-	255	WP_198912806.1	hypothetical protein	-
BRM60_RS14170	2844814..2845104	-	291	WP_027703932.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BRM60_RS14175	2845092..2845370	-	279	WP_011408759.1	CopG family ribbon-helix-helix protein	Antitoxin
BRM60_RS24855	2845451..2845654	-	204	WP_199285669.1	hypothetical protein	-
BRM60_RS24860	2845566..2845910	-	345	WP_199285675.1	hypothetical protein	-
BRM60_RS14185	2845855..2846055	-	201	WP_027703931.1	ribbon-helix-helix protein, CopG family	-
BRM60_RS14190	2846816..2848000	-	1185	WP_011408397.1	IS256 family transposase	-
BRM60_RS14200	2848581..2849546	+	966	WP_109181928.1	IS1595-like element ISXo5 family transposase	-
BRM60_RS14205	2849648..2850253	+	606	Protein_2558	DNA-3-methyladenine glycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10915.48 Da
• Isoelectric Point: 9.5554

>T109222 WP_027703932.1 NZ_CP031456:c2845104-2844814 [Xanthomonas oryzae pv. oryzae]
MPQVIFAPAAIGDMLRLREFLRPKNPDAARRAGEAIRRGVQALGAHPRMGRLIEDLSEQYREWLIDFGDSGYVARYRFDG
DTVTILAVRHQKEAGY

Nucleotide

• Length: 291 bp

>T109222 NZ_CP031456:c2845104-2844814 [Xanthomonas oryzae pv. oryzae]
ATGCCACAAGTAATCTTTGCGCCGGCGGCGATCGGGGACATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGA
TGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGTCCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAG
AAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATTTTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGG
GATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAAGCGGGGTACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10826.11 Da
• Isoelectric Point: 4.9736

>AT109222 WP_011408759.1 NZ_CP031456:c2845370-2845092 [Xanthomonas oryzae pv. oryzae]
MVTATSIKLDDELKGRVQHLAESRRRTPHWIMREAIEQYVEREEKREALNRDTLKAWDEFQATGLHVTAEEVDKWLASWG
TDDELPPPECHK

Nucleotide

• Length: 279 bp

>AT109222 NZ_CP031456:c2845370-2845092 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCTAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A854CIX2