Detailed information of TA system TA109218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 5326570..5327204
• Replicon: chromosome
• Accession: NZ_CP031455
• Organism: Streptomyces olivoreticuli subsp. olivoreticuli strain ATCC 31159

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DXZ73_RS23420
• Protein ID: WP_116213043.1
• Coordinates: 5326570..5326992 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V6K8J5
• Locus tag: DXZ73_RS23425
• Protein ID: WP_023548893.1
• Coordinates: 5326989..5327204 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXZ73_RS23400	5322546..5323589	+	1044	WP_116215790.1	NADP-dependent oxidoreductase	-
DXZ73_RS23405	5323586..5324179	+	594	WP_116213040.1	NAD(P)H oxidoreductase	-
DXZ73_RS23410	5324704..5325615	-	912	WP_162890301.1	DNA polymerase III	-
DXZ73_RS38290	5325910..5326125	-	216	WP_162890302.1	hypothetical protein	-
DXZ73_RS23415	5326143..5326364	-	222	WP_162890303.1	hypothetical protein	-
DXZ73_RS23420	5326570..5326992	-	423	WP_116213043.1	PIN domain nuclease	Toxin
DXZ73_RS23425	5326989..5327204	-	216	WP_023548893.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DXZ73_RS23430	5327297..5327713	-	417	Protein_4575	hypothetical protein	-
DXZ73_RS38295	5328396..5329385	-	990	WP_162890304.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-46)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15440.33 Da
• Isoelectric Point: 4.9090

>T109218 WP_116213043.1 NZ_CP031455:c5326992-5326570 [Streptomyces olivoreticuli subsp. olivoreticuli]
VNAAQFLIDTSALARFMRGDAEQYGWDQAAAAGLIAACPITELEFFYSARSSADRARGIEDMRLIFGWVPVDDRSYDRAW
QVQEALTKQGKHRSAGAVDLLIAATAELQGLTLLHRDHDFECIAAVTGQALQWYGPEHGK

Nucleotide

• Length: 423 bp

>T109218 NZ_CP031455:c5326992-5326570 [Streptomyces olivoreticuli subsp. olivoreticuli]
GTGAACGCGGCCCAGTTCCTGATCGACACCAGCGCGCTCGCGCGGTTCATGCGCGGGGACGCGGAGCAGTACGGCTGGGA
CCAAGCCGCCGCGGCTGGACTCATCGCGGCCTGCCCGATCACCGAGCTCGAGTTCTTCTACAGCGCCCGGTCGTCCGCCG
ACCGGGCGCGCGGCATCGAGGACATGCGGCTGATCTTCGGCTGGGTTCCCGTCGACGACCGCTCCTACGACCGTGCCTGG
CAGGTCCAGGAAGCCCTCACCAAGCAGGGGAAACACCGAAGTGCCGGAGCAGTCGACTTGCTCATCGCCGCGACCGCTGA
GCTGCAGGGGCTCACCCTGCTCCATCGCGATCACGACTTCGAGTGCATCGCCGCAGTGACCGGGCAAGCCCTCCAGTGGT
ACGGCCCGGAACACGGCAAGTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7907.94 Da
• Isoelectric Point: 6.3273

>AT109218 WP_023548893.1 NZ_CP031455:c5327204-5326989 [Streptomyces olivoreticuli subsp. olivoreticuli]
MSRTVIDLDDDLVADVAKALGTSTKKETVNTALREVLESRRRALALARLRAAAGDGAFDLELFENKENYRR

Nucleotide

• Length: 216 bp

>AT109218 NZ_CP031455:c5327204-5326989 [Streptomyces olivoreticuli subsp. olivoreticuli]
ATGAGTCGGACGGTTATCGACCTGGATGACGACCTGGTCGCGGACGTGGCGAAAGCTCTCGGGACGAGCACCAAGAAGGA
AACCGTCAACACCGCTCTTCGCGAGGTGTTGGAGAGCCGGCGTCGCGCCCTGGCTCTCGCCCGGCTGCGGGCCGCGGCGG
GCGACGGCGCATTTGATCTGGAGCTGTTCGAGAACAAGGAGAACTACCGCCGGTGA

Structures

Antitoxin

• AlphaFold DB: V6K8J5