Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1591242..1591858 Replicon chromosome
Accession NZ_CP031450
Organism Pseudomonas fluorescens strain SIK_W1

Toxin (Protein)


Gene name hicA Uniprot ID A0A2T4FRB4
Locus tag DXV65_RS07275 Protein ID WP_065899432.1
Coordinates 1591676..1591858 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2T4FR72
Locus tag DXV65_RS07270 Protein ID WP_065899433.1
Coordinates 1591242..1591652 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXV65_RS07235 1586528..1587454 + 927 WP_120447827.1 LysR family transcriptional regulator -
DXV65_RS07240 1587467..1587877 + 411 WP_120447828.1 fosfomycin resistance glutathione transferase -
DXV65_RS07245 1587922..1588563 + 642 WP_106804881.1 LysE family translocator -
DXV65_RS07250 1588682..1589209 + 528 WP_016975135.1 DUF4142 domain-containing protein -
DXV65_RS07255 1589263..1589688 - 426 WP_065899436.1 low affinity iron permease family protein -
DXV65_RS07260 1589868..1590692 + 825 WP_120447829.1 GNAT family N-acetyltransferase -
DXV65_RS07265 1590651..1591151 - 501 WP_065899434.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
DXV65_RS07270 1591242..1591652 - 411 WP_065899433.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DXV65_RS07275 1591676..1591858 - 183 WP_065899432.1 type II toxin-antitoxin system HicA family toxin Toxin
DXV65_RS07280 1592027..1592920 - 894 WP_065899431.1 LysR family transcriptional regulator -
DXV65_RS07285 1593016..1594179 + 1164 WP_120447830.1 MFS transporter -
DXV65_RS07290 1594176..1595372 + 1197 WP_120447831.1 MFS transporter -
DXV65_RS07295 1595468..1595773 - 306 WP_120447832.1 metal ABC transporter ATPase -
DXV65_RS07300 1595794..1596318 - 525 WP_120447833.1 DUF3833 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(11-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6850.06 Da        Isoelectric Point: 10.8600

>T109208 WP_065899432.1 NZ_CP031450:c1591858-1591676 [Pseudomonas fluorescens]
VDSRYLMGHIVADGWYLVRIRGSHHHFRHPTKPGLVTIPHPKKDLLDKTAKSILKQALLS

Download         Length: 183 bp

>T109208 NZ_CP031450:c1591858-1591676 [Pseudomonas fluorescens]
GTGGATAGCCGATATTTGATGGGGCACATCGTTGCCGATGGCTGGTATCTGGTCCGCATCAGGGGCAGTCATCATCACTT
CAGGCACCCGACCAAACCGGGGCTGGTAACGATTCCTCATCCAAAGAAGGACCTGCTGGACAAGACCGCCAAGAGCATTC
TCAAACAAGCTCTGCTGAGTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14770.68 Da        Isoelectric Point: 4.4796

>AT109208 WP_065899433.1 NZ_CP031450:c1591652-1591242 [Pseudomonas fluorescens]
MLYPIAISTGDQDHAWGVEVPDIPGCFSAGDDLDDAMAMAREAIEGHFEILAEDGAPIPSAQKVTVHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQEH

Download         Length: 411 bp

>AT109208 NZ_CP031450:c1591652-1591242 [Pseudomonas fluorescens]
ATGCTGTATCCCATCGCAATTTCAACCGGCGACCAAGACCATGCCTGGGGCGTGGAAGTCCCGGATATTCCTGGCTGCTT
CTCGGCAGGCGACGACCTCGATGACGCCATGGCCATGGCGCGTGAAGCCATTGAAGGGCACTTCGAGATCCTTGCCGAAG
ACGGTGCACCGATCCCCAGCGCACAGAAAGTCACCGTGCATGCAGCCAACCCACAGTACGCCGGCTGCACCTGGGCGGTG
GTGGACATTGACGTCACCAAGTACCTGGGCAAGGCGCAAAAGCTCAACATCACCTTGCCGGGCTATTTGCTCAACCGCAT
CGATGAATACGTGCTGCATCACCCCGAGGAAAAAAGCCGCTCGGGTTTCCTCGCGTCGGCCGCGCTCAAAGTGCTGCAGC
AAGAGCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2T4FRB4

Antitoxin

Source ID Structure
AlphaFold DB A0A2T4FR72

References