Detailed information of TA system TA109201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2668736..2669370
• Replicon: chromosome
• Accession: NZ_CP031449
• Organism: Pseudomonas aeruginosa strain 97

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CP913_RS13255
• Protein ID: WP_020308731.1
• Coordinates: 2668966..2669370 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CP913_RS13250
• Protein ID: WP_096862007.1
• Coordinates: 2668736..2668966 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP913_RS13225	2664780..2665766	+	987	WP_175425918.1	hypothetical protein	-
CP913_RS13235	2666061..2666558	-	498	WP_017940311.1	DNA repair protein RadC	-
CP913_RS13240	2666530..2667480	-	951	WP_096862005.1	hydrolase or metal-binding protein	-
CP913_RS13245	2667572..2668579	-	1008	WP_096862006.1	YqaJ viral recombinase family protein	-
CP913_RS13250	2668736..2668966	+	231	WP_096862007.1	antitoxin	Antitoxin
CP913_RS13255	2668966..2669370	+	405	WP_020308731.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CP913_RS13260	2669457..2670425	-	969	WP_096862008.1	DUF932 domain-containing protein	-
CP913_RS13270	2671183..2672405	+	1223	WP_096862009.1	IS3 family transposase	-
CP913_RS13275	2672533..2672805	+	273	WP_096862010.1	CopG family ribbon-helix-helix protein	-
CP913_RS13280	2672793..2673086	+	294	WP_010953434.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	sul1 / aadA11 / qnrVC1 / blaDIM-1	-	2622499..2683476	60977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14995.23 Da
• Isoelectric Point: 7.2774

>T109201 WP_020308731.1 NZ_CP031449:2668966-2669370 [Pseudomonas aeruginosa]
MLKYMLDTNMCIFTIKNRPEQVREAFKRHSGQLSISTVTLMELIYGAEKSANPERNLADVEGFAARLEVLPYDAQAAAHS
GQLRAELARIGKPIGPYDQMIAGHARAQGLILVTNNLREFERVPGLRVEDWVNA

Nucleotide

• Length: 405 bp

>T109201 NZ_CP031449:2668966-2669370 [Pseudomonas aeruginosa]
ATGCTGAAGTACATGCTTGATACCAACATGTGCATTTTCACGATCAAGAATCGCCCTGAGCAGGTGCGGGAAGCCTTCAA
GCGCCATTCAGGCCAGCTGAGCATCAGCACTGTCACCCTGATGGAGCTTATTTACGGCGCCGAGAAGTCCGCTAATCCCG
AGCGTAATCTGGCGGACGTTGAAGGTTTCGCTGCGCGCTTGGAAGTACTGCCTTACGACGCTCAGGCAGCGGCTCATTCC
GGGCAATTGCGAGCTGAGTTGGCTCGTATCGGCAAGCCTATTGGCCCGTATGATCAGATGATTGCAGGACACGCACGTGC
CCAGGGACTGATTCTGGTCACGAATAATCTTCGTGAGTTCGAGCGAGTCCCAGGTTTGCGCGTCGAGGATTGGGTGAACG
CCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8460.46 Da
• Isoelectric Point: 4.2683

>AT109201 WP_096862007.1 NZ_CP031449:2668736-2668966 [Pseudomonas aeruginosa]
MEQGAVFQSNRSQAVRLPKAVALPEDVKRVDVVVVGRARIITPAGEAWDVWFDGEGVTADYMADREQPVDQERDAF

Nucleotide

• Length: 231 bp

>AT109201 NZ_CP031449:2668736-2668966 [Pseudomonas aeruginosa]
ATGGAACAAGGTGCAGTTTTTCAGAGCAACCGCAGTCAGGCGGTGCGATTGCCCAAGGCGGTTGCGCTGCCTGAGGATGT
GAAGCGGGTGGATGTAGTAGTAGTTGGTCGAGCTCGAATCATCACGCCGGCAGGCGAGGCCTGGGACGTCTGGTTCGATG
GCGAAGGTGTGACCGCTGACTACATGGCGGATCGCGAGCAGCCTGTAGATCAAGAGCGGGATGCGTTCTGA