Detailed information of TA system TA109198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 1133209..1134630
• Replicon: chromosome
• Accession: NZ_CP031449
• Organism: Pseudomonas aeruginosa strain 97

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: CP913_RS05580
• Protein ID: WP_025297643.1
• Coordinates: 1133557..1134630 (-)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: -
• Locus tag: CP913_RS05575
• Protein ID: WP_158025315.1
• Coordinates: 1133209..1133502 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP913_RS05530	1128312..1128524	+	213	WP_023128602.1	hypothetical protein	-
CP913_RS05535	1128615..1129070	-	456	WP_025297646.1	helix-turn-helix domain-containing protein	-
CP913_RS05540	1129235..1129630	+	396	WP_023128603.1	hypothetical protein	-
CP913_RS05545	1129673..1130143	+	471	WP_023128604.1	hypothetical protein	-
CP913_RS05550	1130146..1130529	+	384	WP_025297645.1	hypothetical protein	-
CP913_RS05555	1130611..1131189	+	579	WP_025297644.1	hypothetical protein	-
CP913_RS05560	1131520..1131884	+	365	Protein_1072	DNA binding protein	-
CP913_RS05565	1132108..1132302	-	195	WP_023128607.1	OprD family outer membrane porin	-
CP913_RS05570	1132277..1133070	+	794	Protein_1074	transporter substrate-binding domain-containing protein	-
CP913_RS05575	1133209..1133502	-	294	WP_158025315.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
CP913_RS05580	1133557..1134630	-	1074	WP_025297643.1	type II toxin-antitoxin system RnlA family toxin	Toxin
CP913_RS05585	1135345..1135599	+	255	WP_023128610.1	hypothetical protein	-
CP913_RS05595	1135897..1136193	+	297	WP_023128612.1	hypothetical protein	-
CP913_RS05600	1136246..1137829	-	1584	WP_025297642.1	hypothetical protein	-
CP913_RS05605	1138109..1138537	+	429	WP_025297641.1	S24 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1119381..1141526	22145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal (6-87)

Antitoxin

• Antitoxin RnlB/LsoB (2-96)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40381.89 Da
• Isoelectric Point: 6.1830

>T109198 WP_025297643.1 NZ_CP031449:c1134630-1133557 [Pseudomonas aeruginosa]
VTERDYKNINLKRCIIEETTHCFIRDNGLKLRSYGDKPGSSGLRVVFGKAGIEDATLDIFFTNVGASTLTYKVGKNQALG
QQLADALYETIHPDEFVTMNLGIKGVDLDDAEALIKELTESANADIEVASRTSADRKHIWKLKSKKNLDELTVTQHENTH
KLQIQGRPLSCYQQLSYLLTELLEINALECILFKKEENRSEFVCAEVAESVLKERFGDCYANLPQTSRRLLLASLCVRLA
SPALPDYCMLVYPELRTLEGAIKQKLAEKNLSNQEESFGGFFTKTNGQFYLKQQYHADVGDAKLQGHLDNAYTFFNKQRH
GLFHMEEMPSVSRMISNITQAVSLCDEAYDHIKNLYS

Nucleotide

• Length: 1074 bp

>T109198 NZ_CP031449:c1134630-1133557 [Pseudomonas aeruginosa]
GTGACTGAACGCGACTACAAAAACATCAACCTCAAACGATGCATTATCGAGGAAACGACTCATTGCTTCATCCGAGATAA
TGGCCTGAAGCTGCGATCCTATGGTGACAAGCCTGGGAGTTCCGGTCTTCGAGTAGTCTTCGGAAAAGCAGGGATTGAAG
ATGCCACGCTTGATATTTTTTTCACGAATGTCGGTGCGTCTACCCTGACCTACAAGGTGGGCAAGAACCAAGCTCTCGGC
CAGCAGCTAGCGGATGCTCTTTACGAAACAATCCACCCCGATGAATTCGTAACGATGAACCTTGGTATCAAAGGCGTCGA
TCTGGATGATGCCGAAGCCCTTATCAAGGAACTCACTGAGTCAGCCAATGCGGACATTGAGGTTGCTTCGCGCACAAGCG
CTGATCGCAAGCATATTTGGAAGCTCAAGTCTAAAAAGAACTTGGATGAGTTGACTGTCACTCAGCACGAGAACACCCAT
AAACTGCAGATCCAGGGTCGTCCTCTCAGCTGCTATCAGCAGTTGAGCTACCTGCTCACCGAGCTCCTAGAGATCAACGC
ACTCGAATGCATCCTGTTCAAAAAGGAAGAAAATCGCTCGGAGTTCGTTTGTGCCGAGGTCGCGGAAAGCGTACTCAAGG
AAAGGTTTGGCGACTGCTACGCCAACCTGCCACAGACCTCGCGTAGGCTGCTCCTGGCAAGCCTTTGCGTGAGGCTTGCC
AGCCCCGCGCTACCCGACTACTGCATGCTGGTTTATCCGGAACTCCGCACCCTTGAAGGCGCGATCAAACAGAAGCTTGC
AGAAAAAAACCTTTCAAATCAGGAAGAAAGCTTCGGTGGCTTCTTTACCAAGACCAATGGTCAGTTTTATCTGAAGCAGC
AATACCATGCTGATGTCGGCGATGCTAAACTACAGGGTCATCTGGACAACGCTTACACCTTCTTCAACAAGCAACGCCAC
GGATTGTTCCACATGGAAGAGATGCCATCCGTTAGTCGAATGATTAGCAACATCACCCAAGCTGTGTCATTGTGTGATGA
AGCATATGATCATATCAAGAATTTGTATAGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11039.38 Da
• Isoelectric Point: 4.5163

>AT109198 WP_158025315.1 NZ_CP031449:c1133502-1133209 [Pseudomonas aeruginosa]
MTVVTATSYENPLSRLSIIASEMRNTSHSSKEIVLFDLLCSNGEEWNRFVSINYNGTDFEKSTCSIVSKSDIPTDLLETQ
TRFFQIHPQYLLDSVLN

Nucleotide

• Length: 294 bp

>AT109198 NZ_CP031449:c1133502-1133209 [Pseudomonas aeruginosa]
ATGACGGTAGTTACCGCTACCAGTTATGAGAACCCCTTATCTCGTCTTTCTATTATTGCTAGCGAGATGCGCAATACATC
TCACTCCAGCAAGGAGATAGTTCTATTCGATCTACTCTGCTCTAACGGAGAAGAGTGGAATCGTTTCGTCTCCATCAATT
ACAACGGCACCGACTTCGAAAAAAGCACCTGTAGCATTGTCTCCAAGTCCGACATCCCTACAGACCTCTTGGAAACACAA
ACTCGCTTCTTTCAAATCCATCCACAATATCTGCTTGATTCAGTTTTGAATTAA