Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 473536..474173 Replicon chromosome
Accession NZ_CP031424
Organism Bacillus velezensis strain B-4

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag DXX91_RS02470 Protein ID WP_003156187.1
Coordinates 473823..474173 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag DXX91_RS02465 Protein ID WP_003156188.1
Coordinates 473536..473817 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXX91_RS02445 469901..470500 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
DXX91_RS02450 470593..470958 + 366 WP_003156192.1 holo-ACP synthase -
DXX91_RS02455 471123..472130 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
DXX91_RS02460 472247..473416 + 1170 WP_115996126.1 alanine racemase -
DXX91_RS02465 473536..473817 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
DXX91_RS02470 473823..474173 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DXX91_RS02475 474291..475112 + 822 WP_014304404.1 STAS domain-containing protein -
DXX91_RS02480 475117..475482 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
DXX91_RS02485 475485..475886 + 402 WP_003156178.1 anti-sigma regulatory factor -
DXX91_RS02490 475898..476905 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
DXX91_RS02495 476969..477298 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
DXX91_RS02500 477295..477777 + 483 WP_003156173.1 anti-sigma B factor RsbW -
DXX91_RS02505 477743..478531 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
DXX91_RS02510 478531..479133 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T109189 WP_003156187.1 NZ_CP031424:473823-474173 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T109189 NZ_CP031424:473823-474173 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT109189 WP_003156188.1 NZ_CP031424:473536-473817 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT109189 NZ_CP031424:473536-473817 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References