Detailed information of TA system TA109188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 524220..524875
• Replicon: chromosome
• Accession: NZ_CP031422
• Organism: Microbacterium oxydans strain HG3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CVS54_RS02550
• Protein ID: WP_046746834.1
• Coordinates: 524480..524875 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0M3DB22
• Locus tag: CVS54_RS18945
• Protein ID: WP_045262550.1
• Coordinates: 524220..524483 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVS54_RS02530	519375..520979	+	1605	WP_046746831.1	urea amidolyase family protein	-
CVS54_RS02535	520976..522706	+	1731	WP_127011687.1	ATP-grasp domain-containing protein	-
CVS54_RS02540	522780..524108	-	1329	WP_046746833.1	NCS2 family nucleobase:cation symporter	-
CVS54_RS18945	524220..524483	+	264	WP_045262550.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CVS54_RS02550	524480..524875	+	396	WP_046746834.1	type II toxin-antitoxin system VapC family toxin	Toxin
CVS54_RS02555	524894..525772	-	879	WP_127011689.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CVS54_RS02560	525991..526617	+	627	WP_127011690.1	PadR family transcriptional regulator	-
CVS54_RS02565	526614..528185	+	1572	WP_127011691.1	MFS transporter	-
CVS54_RS02570	528252..528806	+	555	WP_127011692.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-86)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 13980.97 Da
• Isoelectric Point: 4.2530

>T109188 WP_046746834.1 NZ_CP031422:524480-524875 [Microbacterium oxydans]
MIVDSSALVAVLMAEPEAAEMSELLEEGPFSLSAATLTECMIVLRGKGGEAKVLALDELLEATGATVVPLDEAQARLAAR
AYVRFGRGSGSAARLNYGDCFSYALAISRDEPLLFKGDDFIHTDVRRVELS

Nucleotide

• Length: 396 bp

>T109188 NZ_CP031422:524480-524875 [Microbacterium oxydans]
TTGATCGTCGATTCCTCGGCCCTCGTGGCTGTCTTGATGGCTGAGCCCGAAGCTGCCGAGATGAGCGAACTGTTGGAGGA
GGGCCCCTTCTCACTGTCCGCGGCCACGCTCACGGAGTGCATGATCGTGCTCCGCGGCAAGGGAGGCGAGGCGAAGGTGC
TCGCGCTGGACGAGCTTCTGGAAGCGACGGGTGCGACCGTCGTTCCGCTCGACGAGGCCCAGGCGCGTCTGGCGGCCAGG
GCGTACGTCAGATTCGGACGCGGTTCCGGGAGCGCGGCTCGGCTGAACTATGGCGACTGCTTCTCTTATGCGCTCGCGAT
CAGTCGCGACGAGCCTCTGCTCTTCAAGGGTGACGACTTCATCCACACAGACGTGCGCCGGGTGGAGCTGAGCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10123.31 Da
• Isoelectric Point: 5.9280

>AT109188 WP_045262550.1 NZ_CP031422:524220-524483 [Microbacterium oxydans]
MSLNIKNETTHELVRRLAALTGQSQTSAVEDAVRRRLAELEEQRSGEEEERRRRIRAVIRRAQQIPSTGRTTEEIMDELY
DETGMPR

Nucleotide

• Length: 264 bp

>AT109188 NZ_CP031422:524220-524483 [Microbacterium oxydans]
ATGAGTCTGAACATCAAGAACGAGACGACGCATGAGCTGGTGCGCCGGCTCGCGGCGCTCACCGGGCAGAGTCAGACCAG
TGCGGTGGAAGATGCCGTTCGGCGGCGCCTCGCCGAGTTGGAGGAGCAACGCTCGGGCGAAGAAGAGGAGCGCCGTCGCC
GTATCCGTGCGGTGATCCGCCGGGCGCAGCAGATTCCCTCCACCGGACGCACGACCGAGGAGATCATGGACGAGCTCTAC
GACGAGACGGGTATGCCGCGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0M3DB22