Detailed information of TA system TA109180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2191597..2192132
• Replicon: chromosome
• Accession: NZ_CP031414
• Organism: Mycolicibacterium neoaurum strain HGMS2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: V5XEF3
• Locus tag: DXK33_RS10360
• Protein ID: WP_023985812.1
• Coordinates: 2191597..2191929 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: V5XDA1
• Locus tag: DXK33_RS10365
• Protein ID: WP_019512785.1
• Coordinates: 2191926..2192132 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXK33_RS10335	2186969..2187976	-	1008	WP_019512779.1	amidohydrolase	-
DXK33_RS10340	2188039..2188473	+	435	WP_019512780.1	MarR family winged helix-turn-helix transcriptional regulator	-
DXK33_RS10345	2188603..2189175	+	573	WP_019512781.1	TetR/AcrR family transcriptional regulator	-
DXK33_RS10350	2189237..2190163	+	927	WP_019512782.1	class I SAM-dependent methyltransferase	-
DXK33_RS10355	2190622..2191332	-	711	WP_131701367.1	hypothetical protein	-
DXK33_RS10360	2191597..2191929	-	333	WP_023985812.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DXK33_RS10365	2191926..2192132	-	207	WP_019512785.1	antitoxin MazE family protein	Antitoxin
DXK33_RS10375	2192502..2193275	-	774	WP_023985811.1	HAD-IIA family hydrolase	-
DXK33_RS10380	2193697..2194284	-	588	WP_019512788.1	TetR/AcrR family transcriptional regulator	-
DXK33_RS10385	2194379..2196361	+	1983	WP_019512789.1	FAD-dependent monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2196424..2197281	857

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin MazE-like (4-66)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11770.61 Da
• Isoelectric Point: 6.4638

>T109180 WP_023985812.1 NZ_CP031414:c2191929-2191597 [Mycolicibacterium neoaurum]
VNRGEIWTVAGGVYAAKPRPAVIVQDDLFDATSSVTVAPMTSTLLDAPLMRIRIAGGADLLSGLDHDSDVMIDKLTTVRK
SNVHARVGRLTAEQVVEVERALMAFLGLAR

Nucleotide

• Length: 333 bp

>T109180 NZ_CP031414:c2191929-2191597 [Mycolicibacterium neoaurum]
GTGAACCGAGGAGAGATCTGGACCGTAGCGGGGGGCGTCTATGCAGCGAAGCCGCGTCCGGCAGTGATTGTTCAGGATGA
CCTCTTCGACGCGACAAGTTCAGTGACCGTGGCGCCGATGACCAGCACGCTGTTGGATGCACCTTTGATGCGCATTCGGA
TAGCCGGCGGAGCAGACCTGCTGTCCGGACTTGACCACGACAGTGACGTGATGATCGACAAGCTCACCACCGTGAGAAAA
TCGAATGTCCATGCTCGTGTCGGCCGTCTGACTGCCGAGCAAGTGGTCGAGGTCGAGCGAGCCTTGATGGCGTTCCTCGG
CCTCGCGCGATGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7924.77 Da
• Isoelectric Point: 4.4805

>AT109180 WP_019512785.1 NZ_CP031414:c2192132-2191926 [Mycolicibacterium neoaurum]
VAVRERVGEYRRRMRERGLRPLQVWVPDVRTETFAAEAHRQASLVAQAEDVSDDQDFVEAISVPWDEE

Nucleotide

• Length: 207 bp

>AT109180 NZ_CP031414:c2192132-2191926 [Mycolicibacterium neoaurum]
GTGGCGGTGCGGGAACGGGTTGGCGAGTATCGCCGGCGGATGCGGGAGCGGGGCTTGCGACCTCTGCAGGTCTGGGTGCC
TGATGTGCGGACGGAGACCTTCGCGGCCGAGGCTCACCGTCAGGCGTCGTTGGTTGCGCAAGCCGAAGACGTCAGCGACG
ACCAGGATTTCGTTGAGGCGATCTCGGTGCCTTGGGACGAGGAGTGA

Structures

Toxin

• AlphaFold DB: V5XEF3

Antitoxin

• AlphaFold DB: V5XDA1