Detailed information of TA system TA109148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-couple_hipB
• Location: 983742..984985
• Replicon: chromosome
• Accession: NZ_CP031377
• Organism: Streptococcus suis strain ISU2614

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: G7SEB4
• Locus tag: DK237_RS05050
• Protein ID: WP_001235242.1
• Coordinates: 983742..984509 (-)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: sezA
• Uniprot ID: -
• Locus tag: DK237_RS05055
• Protein ID: WP_000578719.1
• Coordinates: 984509..984985 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DK237_RS05025	979107..981599	-	2493	WP_079397378.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
DK237_RS05030	981745..982482	-	738	WP_001038796.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
DK237_RS05035	982607..982687	-	81	WP_032501554.1	23S rRNA methyltransferase attenuator leader peptide ErmL	-
DK237_RS05040	982753..982992	-	240	WP_002425012.1	peptide-binding protein	-
DK237_RS05045	983112..983663	-	552	Protein_961	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
DK237_RS05050	983742..984509	-	768	WP_001235242.1	zeta toxin family protein	Toxin
DK237_RS05055	984509..984985	-	477	WP_000578719.1	helix-turn-helix domain-containing protein	Antitoxin
DK237_RS05060	985055..985345	-	291	WP_000667951.1	hypothetical protein	-
DK237_RS05065	985396..985785	-	390	WP_000048066.1	hypothetical protein	-
DK237_RS05070	985782..986009	-	228	WP_000110922.1	hypothetical protein	-
DK237_RS05075	986037..987137	-	1101	WP_000196860.1	toprim domain-containing protein	-
DK237_RS05080	987177..987815	-	639	WP_053861411.1	hypothetical protein	-
DK237_RS05085	987910..988200	-	291	WP_000893083.1	hypothetical protein	-
DK237_RS05090	988214..988513	-	300	WP_000169471.1	hypothetical protein	-
DK237_RS05095	988584..989543	-	960	Protein_971	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / tet(O)	-	959499..1024540	65041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 28985.80 Da
• Isoelectric Point: 5.1126

>T109148 WP_001235242.1 NZ_CP031377:c984509-983742 [Streptococcus suis]
MRLEEFSEAEFQKALQRTIRALTRGKTIPDQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEQLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLSSKGYQVSLAVIGTKPELSYLSTLIRYEE
LYAIDPTQARATPKEHHDGIVENLVDNLRELESENLFDQIQIYQRDRTCIYDSETAEGSAAEVLQECLFGKWSKVEEEMV
KESELLLKELLEKNK

Nucleotide

• Length: 768 bp

>T109148 NZ_CP031377:c984509-983742 [Streptococcus suis]
ATGAGACTGGAAGAATTTAGTGAGGCTGAGTTTCAGAAGGCCTTGCAGCGAACCATTCGGGCCTTAACCCGAGGAAAGAC
GATTCCAGATCAACCGAAAGCTATCTTGCTTGGTGGACAAAGCGGAGCAGGTAAGACGACTATTCATCGTATCAAGCAAA
AGGAATTTCAAGGTAATATCATTATTATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGCCCTGCAAGAA
AAGTATGGCAAGGATAGCGTGGACTATACCAAGGGATTTGCAGGAAAAATGGTAGAACAGTTGGTTGATGAACTCAGTAC
GCAAGGCTACCATTTGCTGATTGAGGGAACCTTGCGAACTACTCAAGTTCCTCGTCAGACTGCTCAATTATTATCTTCTA
AAGGTTACCAAGTTTCCTTAGCAGTGATTGGTACCAAACCAGAGCTTTCTTATCTCAGCACCTTGATTCGTTACGAAGAG
CTTTATGCCATCGATCCGACTCAGGCTAGAGCAACACCAAAAGAACACCACGATGGGATTGTTGAGAACTTGGTTGATAA
CTTGAGGGAGTTAGAAAGTGAAAATCTCTTTGACCAGATTCAAATTTATCAAAGAGATAGAACTTGTATCTATGATTCTG
AAACTGCTGAAGGTTCCGCAGCAGAAGTGCTACAAGAATGCCTATTTGGAAAATGGAGCAAGGTAGAGGAGGAGATGGTG
AAGGAGAGTGAACTCCTACTTAAGGAATTACTTGAGAAAAATAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18266.77 Da
• Isoelectric Point: 4.6424

>AT109148 WP_000578719.1 NZ_CP031377:c984985-984509 [Streptococcus suis]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLTPVEDYHLTLKVE
VIKERGAAILSKLYRYQDSQDIVFDDESNPWILMSDDLAELINTKIYLVATFDEIERYNGYLDGIERMLDMVHHRVVA

Nucleotide

• Length: 477 bp

>AT109148 NZ_CP031377:c984985-984509 [Streptococcus suis]
ATGATCGGAGACAATATAAAATCACTACGCCGAACACACGATTTAACACAGCCAGAATTTGCGAAAATGGTTGGGATTTC
ACGCAATAGCCTGAGTCGCTACGAAAATGGTACCAGCACTGTTTCAACTGAACTCATCGACCGTATTTGTCAGAAATTTA
ACGTTTCTTATGTCGATATTGTAGGAGAGGACAAGATGTTAACACCTGTCGAAGATTACCATCTGACTTTAAAAGTAGAA
GTGATAAAAGAACGTGGAGCAGCCATTTTATCAAAGCTTTATAGATACCAGGATAGTCAAGATATTGTTTTTGATGATGA
ATCTAATCCTTGGATTTTGATGAGTGATGACTTGGCCGAATTGATCAATACGAAAATTTACCTTGTCGCTACTTTCGATG
AAATAGAGCGTTACAATGGCTATTTAGATGGTATAGAGCGGATGTTAGACATGGTGCATCATCGGGTGGTGGCTTAA

Structures

Toxin

• AlphaFold DB: G7SEB4