Detailed information of TA system TA109141

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5121675..5122507
• Replicon: chromosome
• Accession: NZ_CP031355
• Organism: Escherichia coli O145 strain RM8995-C1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: DXE49_RS25765
• Protein ID: WP_000854753.1
• Coordinates: 5122133..5122507 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9QGA8
• Locus tag: DXE49_RS25760
• Protein ID: WP_001280948.1
• Coordinates: 5121675..5122043 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXE49_RS25720	5116880..5117302	+	423	Protein_4935	hypothetical protein	-
DXE49_RS25730	5118595..5118966	+	372	WP_064758448.1	hypothetical protein	-
DXE49_RS25735	5119045..5119278	+	234	WP_001119729.1	DUF905 family protein	-
DXE49_RS25740	5119378..5120196	+	819	WP_001234620.1	DUF945 domain-containing protein	-
DXE49_RS25745	5120251..5120736	+	486	WP_000849571.1	antirestriction protein	-
DXE49_RS25750	5120752..5121228	+	477	WP_001186726.1	RadC family protein	-
DXE49_RS25755	5121291..5121512	+	222	WP_000692311.1	DUF987 domain-containing protein	-
DXE49_RS25760	5121675..5122043	+	369	WP_001280948.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DXE49_RS25765	5122133..5122507	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
DXE49_RS25770	5122504..5122992	+	489	WP_000777552.1	hypothetical protein	-
DXE49_RS25775	5123004..5123201	+	198	WP_000839281.1	DUF957 domain-containing protein	-
DXE49_RS25780	5123298..5123858	+	561	WP_044869391.1	DUF4942 domain-containing protein	-
DXE49_RS25785	5123884..5125097	+	1214	WP_136952279.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	efa1 / efa1 / nleE / nleB1 / espL2	5117344..5142899	25555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T109141 WP_000854753.1 NZ_CP031355:5122133-5122507 [Escherichia coli O145]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T109141 NZ_CP031355:5122133-5122507 [Escherichia coli O145]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13609.50 Da
• Isoelectric Point: 7.3991

>AT109141 WP_001280948.1 NZ_CP031355:5121675-5122043 [Escherichia coli O145]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT109141 NZ_CP031355:5121675-5122043 [Escherichia coli O145]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: A0A7U9QGA8