Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5081527..5082024 Replicon chromosome
Accession NZ_CP031355
Organism Escherichia coli O145 strain RM8995-C1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag DXE49_RS25580 Protein ID WP_001173343.1
Coordinates 5081851..5082024 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag DXE49_RS25575 Protein ID WP_000398619.1
Coordinates 5081527..5081823 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE49_RS25555 5078258..5078977 - 720 WP_000611288.1 two-component system response regulator DcuR -
DXE49_RS25560 5078974..5080605 - 1632 WP_022581427.1 sensor histidine kinase -
DXE49_RS25565 5080786..5081016 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
DXE49_RS25570 5081028..5081300 + 273 WP_000405653.1 N-acetyltransferase -
DXE49_RS25575 5081527..5081823 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
DXE49_RS25580 5081851..5082024 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
DXE49_RS25585 5082143..5083660 - 1518 WP_001295074.1 lysine--tRNA ligase -
DXE49_RS25590 5083897..5085354 - 1458 WP_000856822.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T109140 WP_001173343.1 NZ_CP031355:5081851-5082024 [Escherichia coli O145]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T109140 NZ_CP031355:5081851-5082024 [Escherichia coli O145]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT109140 WP_000398619.1 NZ_CP031355:5081527-5081823 [Escherichia coli O145]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT109140 NZ_CP031355:5081527-5081823 [Escherichia coli O145]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References