Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4261057..4261688 Replicon chromosome
Accession NZ_CP031355
Organism Escherichia coli O145 strain RM8995-C1

Toxin (Protein)


Gene name hicA Uniprot ID A0A7U9LJQ2
Locus tag DXE49_RS21745 Protein ID WP_001259387.1
Coordinates 4261413..4261688 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag DXE49_RS21740 Protein ID WP_000593555.1
Coordinates 4261057..4261416 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE49_RS21710 4256169..4256642 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
DXE49_RS21715 4256639..4256920 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
DXE49_RS21720 4256997..4258355 + 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
DXE49_RS21725 4258348..4259856 + 1509 WP_001400448.1 carbohydrate kinase -
DXE49_RS21730 4259846..4260115 + 270 WP_000933061.1 HPr family phosphocarrier protein -
DXE49_RS21735 4260147..4261007 + 861 WP_001131788.1 class II aldolase -
DXE49_RS21740 4261057..4261416 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DXE49_RS21745 4261413..4261688 - 276 WP_001259387.1 type II toxin-antitoxin system HicA family toxin Toxin
DXE49_RS21750 4261761..4262885 - 1125 WP_001314210.1 ABC transporter permease -
DXE49_RS21755 4262885..4265620 - 2736 WP_000149086.1 ribosome-associated ATPase/putative transporter RbbA -
DXE49_RS21760 4265617..4266684 - 1068 WP_000361517.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4255398..4270523 15125


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10214.91 Da        Isoelectric Point: 10.7076

>T109139 WP_001259387.1 NZ_CP031355:c4261688-4261413 [Escherichia coli O145]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T109139 NZ_CP031355:c4261688-4261413 [Escherichia coli O145]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT109139 WP_000593555.1 NZ_CP031355:c4261416-4261057 [Escherichia coli O145]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT109139 NZ_CP031355:c4261416-4261057 [Escherichia coli O145]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAGCTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LJQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References