Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3652999..3653653 Replicon chromosome
Accession NZ_CP031355
Organism Escherichia coli O145 strain RM8995-C1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag DXE49_RS18595 Protein ID WP_000244777.1
Coordinates 3652999..3653406 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag DXE49_RS18600 Protein ID WP_000354046.1
Coordinates 3653387..3653653 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE49_RS18575 3648956..3650689 - 1734 WP_000813202.1 single-stranded-DNA-specific exonuclease RecJ -
DXE49_RS18580 3650695..3651405 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
DXE49_RS18585 3651430..3652326 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
DXE49_RS18590 3652438..3652959 + 522 WP_001055874.1 flavodoxin FldB -
DXE49_RS18595 3652999..3653406 - 408 WP_000244777.1 toxin CptA Toxin
DXE49_RS18600 3653387..3653653 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
DXE49_RS18605 3653895..3654875 + 981 WP_000886093.1 tRNA-modifying protein YgfZ -
DXE49_RS18610 3654952..3655611 - 660 WP_000250294.1 hemolysin III family protein -
DXE49_RS18615 3655775..3656086 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
DXE49_RS18620 3656131..3657564 + 1434 WP_024221758.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T109134 WP_000244777.1 NZ_CP031355:c3653406-3652999 [Escherichia coli O145]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T109134 NZ_CP031355:c3653406-3652999 [Escherichia coli O145]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT109134 WP_000354046.1 NZ_CP031355:c3653653-3653387 [Escherichia coli O145]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT109134 NZ_CP031355:c3653653-3653387 [Escherichia coli O145]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References