Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 78280..78923 Replicon plasmid pRM8995-C1
Accession NZ_CP031354
Organism Escherichia coli O145 strain RM8995-C1

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag DXE49_RS00395 Protein ID WP_001044768.1
Coordinates 78280..78696 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag DXE49_RS00400 Protein ID WP_001261287.1
Coordinates 78693..78923 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE49_RS00370 74825..75172 - 348 WP_000631725.1 IS66 family insertion sequence element accessory protein TnpB -
DXE49_RS00375 75169..75843 - 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -
DXE49_RS00380 75897..76151 - 255 WP_097455692.1 tyrosine-type recombinase/integrase -
DXE49_RS00390 77573..78118 + 546 WP_001165114.1 hypothetical protein -
DXE49_RS00395 78280..78696 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
DXE49_RS00400 78693..78923 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DXE49_RS00405 79483..79896 + 414 WP_000465041.1 hypothetical protein -
DXE49_RS00410 79898..80680 + 783 WP_001164205.1 site-specific integrase -
DXE49_RS00415 80853..81206 + 354 WP_000864810.1 colicin M immunity protein -
DXE49_RS00420 81214..81405 - 192 Protein_83 lipid II-degrading bacteriocin -
DXE49_RS00425 81409..81552 - 144 Protein_84 transcriptional regulator -
DXE49_RS00430 81619..83058 - 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - espP / toxB / toxB / toxB / hlyD / hlyB / hlyA / hlyC 1..88747 88747


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T109117 WP_001044768.1 NZ_CP031354:c78696-78280 [Escherichia coli O145]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T109117 NZ_CP031354:c78696-78280 [Escherichia coli O145]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT109117 WP_001261287.1 NZ_CP031354:c78923-78693 [Escherichia coli O145]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT109117 NZ_CP031354:c78923-78693 [Escherichia coli O145]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References