Detailed information of TA system TA109082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 54753..55303
• Replicon: chromosome
• Accession: NZ_CP031353
• Organism: Escherichia coli O145 strain RM9154-C1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: Q32K46
• Locus tag: DXE50_RS02255
• Protein ID: WP_001160974.1
• Coordinates: 54989..55303 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: DXE50_RS02250
• Protein ID: WP_096846550.1
• Coordinates: 54753..54986 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXE50_RS02225	49825..50112	+	288	WP_000203748.1	ferredoxin-like protein FixX	-
DXE50_RS02230	50170..51501	+	1332	WP_001183192.1	MFS transporter	-
DXE50_RS02235	51612..52142	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
DXE50_RS02240	52135..53997	+	1863	WP_000377137.1	glutathione-regulated potassium-efflux system protein KefC	-
DXE50_RS02245	54188..54667	+	480	WP_024221573.1	type 3 dihydrofolate reductase	-
DXE50_RS02250	54753..54986	+	234	WP_096846550.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
DXE50_RS02255	54989..55303	+	315	WP_001160974.1	CcdB family protein	Toxin
DXE50_RS02260	55300..56148	-	849	WP_000257203.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
DXE50_RS02265	56155..56532	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
DXE50_RS02270	56535..57356	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
DXE50_RS02275	57353..58342	-	990	WP_000241240.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
DXE50_RS02280	58342..59628	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11737.84 Da
• Isoelectric Point: 9.8517

>T109082 WP_001160974.1 NZ_CP031353:54989-55303 [Escherichia coli O145]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T109082 NZ_CP031353:54989-55303 [Escherichia coli O145]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8477.48 Da
• Isoelectric Point: 5.0348

>AT109082 WP_096846550.1 NZ_CP031353:54753-54986 [Escherichia coli O145]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATCWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT109082 NZ_CP031353:54753-54986 [Escherichia coli O145]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACATGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A7U9LRR3