Detailed information of TA system TA109074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4156205..4157005
• Replicon: chromosome
• Accession: NZ_CP031349
• Organism: Escherichia coli O145 strain RM9467-C1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: U9Y257
• Locus tag: DXE51_RS20990
• Protein ID: WP_000342447.1
• Coordinates: 4156205..4156732 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: U9Y285
• Locus tag: DXE51_RS20995
• Protein ID: WP_001277109.1
• Coordinates: 4156739..4157005 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXE51_RS20965	4151279..4152046	-	768	WP_000082096.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
DXE51_RS20970	4152043..4153320	-	1278	WP_000803782.1	branched chain amino acid ABC transporter permease LivM	-
DXE51_RS20975	4153317..4154243	-	927	WP_000003013.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
DXE51_RS20980	4154291..4155400	-	1110	WP_001400431.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
DXE51_RS20985	4155825..4156208	+	384	WP_000778795.1	aspartate 1-decarboxylase autocleavage activator PanM	-
DXE51_RS20990	4156205..4156732	-	528	WP_000342447.1	GNAT family N-acetyltransferase	Toxin
DXE51_RS20995	4156739..4157005	-	267	WP_001277109.1	DUF1778 domain-containing protein	Antitoxin
DXE51_RS21000	4157155..4158258	-	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
DXE51_RS21005	4158529..4159383	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
DXE51_RS21010	4159628..4160686	-	1059	WP_001042003.1	permease-like cell division protein FtsX	-
DXE51_RS21015	4160679..4161347	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.63 Da
• Isoelectric Point: 7.0284

>T109074 WP_000342447.1 NZ_CP031349:c4156732-4156205 [Escherichia coli O145]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDHSIQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T109074 NZ_CP031349:c4156732-4156205 [Escherichia coli O145]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTTTGGCAATTGATCATTCAATACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCAGTAGGTATTCACGGTCTTTTTGTAG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCGTTGGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9938.37 Da
• Isoelectric Point: 10.0863

>AT109074 WP_001277109.1 NZ_CP031349:c4157005-4156739 [Escherichia coli O145]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Nucleotide

• Length: 267 bp

>AT109074 NZ_CP031349:c4157005-4156739 [Escherichia coli O145]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGTAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LUM2

Antitoxin

• AlphaFold DB: A0A7U9LUN0