Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1913698..1914336 Replicon chromosome
Accession NZ_CP031349
Organism Escherichia coli O145 strain RM9467-C1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag DXE51_RS09800 Protein ID WP_000813794.1
Coordinates 1914160..1914336 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag DXE51_RS09795 Protein ID WP_001270286.1
Coordinates 1913698..1914114 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE51_RS09775 1908849..1909790 - 942 WP_001251328.1 ABC transporter permease -
DXE51_RS09780 1909791..1910804 - 1014 WP_000220414.1 ABC transporter ATP-binding protein -
DXE51_RS09785 1910822..1911967 - 1146 WP_000335342.1 ABC transporter substrate-binding protein -
DXE51_RS09790 1912213..1913619 - 1407 WP_000760665.1 PLP-dependent aminotransferase family protein -
DXE51_RS09795 1913698..1914114 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
DXE51_RS09800 1914160..1914336 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
DXE51_RS09805 1914558..1914788 + 231 WP_000494244.1 YncJ family protein -
DXE51_RS09810 1914880..1916841 - 1962 WP_000218823.1 23S rRNA 5-hydroxycytidine C2501 synthase -
DXE51_RS09815 1916914..1917450 - 537 WP_000429147.1 DNA-binding transcriptional regulator SutR -
DXE51_RS09820 1917503..1918714 + 1212 WP_071792349.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T109058 WP_000813794.1 NZ_CP031349:c1914336-1914160 [Escherichia coli O145]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T109058 NZ_CP031349:c1914336-1914160 [Escherichia coli O145]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT109058 WP_001270286.1 NZ_CP031349:c1914114-1913698 [Escherichia coli O145]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT109058 NZ_CP031349:c1914114-1913698 [Escherichia coli O145]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References