Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/-
Location 4285697..4286328 Replicon chromosome
Accession NZ_CP031345
Organism Escherichia coli O145 strain RM12367-C1

Toxin (Protein)


Gene name hicA Uniprot ID A0A7U9LJQ2
Locus tag DXE53_RS21870 Protein ID WP_001259387.1
Coordinates 4286053..4286328 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag DXE53_RS21865 Protein ID WP_000593555.1
Coordinates 4285697..4286056 (-) Length 120 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE53_RS21835 4280809..4281282 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
DXE53_RS21840 4281279..4281560 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
DXE53_RS21845 4281637..4282995 + 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
DXE53_RS21850 4282988..4284496 + 1509 WP_001400448.1 carbohydrate kinase -
DXE53_RS21855 4284486..4284755 + 270 WP_000933061.1 HPr family phosphocarrier protein -
DXE53_RS21860 4284787..4285647 + 861 WP_001131788.1 class II aldolase -
DXE53_RS21865 4285697..4286056 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DXE53_RS21870 4286053..4286328 - 276 WP_001259387.1 type II toxin-antitoxin system HicA family toxin Toxin
DXE53_RS21875 4286401..4287525 - 1125 WP_001314210.1 ABC transporter permease -
DXE53_RS21880 4287525..4290260 - 2736 WP_000149086.1 ribosome-associated ATPase/putative transporter RbbA -
DXE53_RS21885 4290257..4291324 - 1068 WP_000361517.1 HlyD family secretion protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(30-80)

Antitoxin

(60-112)


Sequences


Toxin        


Download         Length: 92 a.a.        Molecular weight: 10214.91 Da        Isoelectric Point: 10.7076

>T109017 WP_001259387.1 NZ_CP031345:c4286328-4286053 [Escherichia coli O145]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T109017 NZ_CP031345:c4286328-4286053 [Escherichia coli O145]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT109017 WP_000593555.1 NZ_CP031345:c4286056-4285697 [Escherichia coli O145]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT109017 NZ_CP031345:c4286056-4285697 [Escherichia coli O145]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAGCTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LJQ2


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References