Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1759735..1760373 Replicon chromosome
Accession NZ_CP031343
Organism Escherichia coli O145 strain RM10425-C1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag DXE54_RS09125 Protein ID WP_000813794.1
Coordinates 1759735..1759911 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag DXE54_RS09130 Protein ID WP_001270286.1
Coordinates 1759957..1760373 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE54_RS09105 1755357..1756568 - 1212 WP_071792349.1 BenE family transporter YdcO -
DXE54_RS09110 1756621..1757157 + 537 WP_000429147.1 DNA-binding transcriptional regulator SutR -
DXE54_RS09115 1757230..1759191 + 1962 WP_000218823.1 23S rRNA 5-hydroxycytidine C2501 synthase -
DXE54_RS09120 1759283..1759513 - 231 WP_000494244.1 YncJ family protein -
DXE54_RS09125 1759735..1759911 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
DXE54_RS09130 1759957..1760373 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
DXE54_RS09135 1760452..1761858 + 1407 WP_000760665.1 PLP-dependent aminotransferase family protein -
DXE54_RS09140 1762104..1763249 + 1146 WP_000335342.1 ABC transporter substrate-binding protein -
DXE54_RS09145 1763267..1764280 + 1014 WP_000220414.1 ABC transporter ATP-binding protein -
DXE54_RS09150 1764281..1765222 + 942 WP_001251328.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T108977 WP_000813794.1 NZ_CP031343:1759735-1759911 [Escherichia coli O145]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T108977 NZ_CP031343:1759735-1759911 [Escherichia coli O145]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT108977 WP_001270286.1 NZ_CP031343:1759957-1760373 [Escherichia coli O145]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT108977 NZ_CP031343:1759957-1760373 [Escherichia coli O145]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References