Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4261012..4261643 Replicon chromosome
Accession NZ_CP031341
Organism Escherichia coli O145 strain RM12275-C1

Toxin (Protein)


Gene name hicA Uniprot ID A0A7U9LJQ2
Locus tag DXE55_RS21735 Protein ID WP_001259387.1
Coordinates 4261368..4261643 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag DXE55_RS21730 Protein ID WP_000593555.1
Coordinates 4261012..4261371 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE55_RS21700 4256124..4256597 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
DXE55_RS21705 4256594..4256875 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
DXE55_RS21710 4256952..4258310 + 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
DXE55_RS21715 4258303..4259811 + 1509 WP_001400448.1 carbohydrate kinase -
DXE55_RS21720 4259801..4260070 + 270 WP_000933061.1 HPr family phosphocarrier protein -
DXE55_RS21725 4260102..4260962 + 861 WP_001131788.1 class II aldolase -
DXE55_RS21730 4261012..4261371 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DXE55_RS21735 4261368..4261643 - 276 WP_001259387.1 type II toxin-antitoxin system HicA family toxin Toxin
DXE55_RS21740 4261716..4262840 - 1125 WP_001314210.1 ABC transporter permease -
DXE55_RS21745 4262840..4265575 - 2736 WP_000149086.1 ribosome-associated ATPase/putative transporter RbbA -
DXE55_RS21750 4265572..4266639 - 1068 WP_000361517.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4255353..4270478 15125


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10214.91 Da        Isoelectric Point: 10.7076

>T108963 WP_001259387.1 NZ_CP031341:c4261643-4261368 [Escherichia coli O145]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T108963 NZ_CP031341:c4261643-4261368 [Escherichia coli O145]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT108963 WP_000593555.1 NZ_CP031341:c4261371-4261012 [Escherichia coli O145]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT108963 NZ_CP031341:c4261371-4261012 [Escherichia coli O145]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAGCTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LJQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References