Detailed information of TA system TA108931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 65162..65697
• Replicon: plasmid pEsST2350_SE_2
• Accession: NZ_CP031323
• Organism: Escherichia coli strain ST2350 isolate Es_ST2350_SE1_NDM_03_2018

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DW289_RS25135
• Protein ID: WP_089584420.1
• Coordinates: 65410..65697 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1PXV2
• Locus tag: DW289_RS25130
• Protein ID: WP_001139206.1
• Coordinates: 65162..65413 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DW289_RS25090	60175..61351	+	1177	Protein_70	IS3 family transposase	-
DW289_RS25095	61712..62014	+	303	WP_001346256.1	DUF1778 domain-containing protein	-
DW289_RS25100	62005..62487	+	483	WP_000405244.1	GNAT family N-acetyltransferase	-
DW289_RS25105	62797..63054	+	258	WP_000083825.1	replication regulatory protein RepA	-
DW289_RS25690	63158..63356	+	199	Protein_74	protein CopA/IncA	-
DW289_RS25120	63353..64210	+	858	WP_122990696.1	incFII family plasmid replication initiator RepA	-
DW289_RS25130	65162..65413	+	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DW289_RS25135	65410..65697	+	288	WP_089584420.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DW289_RS25140	65987..66187	-	201	WP_089584419.1	alkaline phosphatase	-
DW289_RS25145	66177..66382	+	206	Protein_79	hypothetical protein	-
DW289_RS25150	66557..70054	-	3498	WP_115431762.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	paa / papH / papC / papD	1..82461	82461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11144.19 Da
• Isoelectric Point: 10.6261

>T108931 WP_089584420.1 NZ_CP031323:65410-65697 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKAIQQQFAKKLKKCSENPHIASARLRGLKDCYKIKLRTSGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Nucleotide

• Length: 288 bp

>T108931 NZ_CP031323:65410-65697 [Escherichia coli]
ATGACTTATACGGTAAAATTCCGGGATGATGCGCTGAAAGAATGGCTCAGACTGGATAAGGCCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCCCATATAGCTTCTGCAAGACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCACATCAGGTTTTCGTCTTGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTAGTCGCAGTAGGTAAA
CGTGAACGTAGCAACGTTTATCATCTCGCCAGTGAGCGGATGAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9171.39 Da
• Isoelectric Point: 4.0927

>AT108931 WP_001139206.1 NZ_CP031323:65162-65413 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT108931 NZ_CP031323:65162-65413 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGTTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCAGCTGAGCTCTATGAAAGAATGC
TTGATGCCCTGGATGACCAGGAGCTGGTGAAACTGGTTACTGAGCGCAGCAGCCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Antitoxin

• AlphaFold DB: T1PXV2