Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 61712..62487 Replicon plasmid pEsST2350_SE_2
Accession NZ_CP031323
Organism Escherichia coli strain ST2350 isolate Es_ST2350_SE1_NDM_03_2018

Toxin (Protein)


Gene name tacT Uniprot ID A0A8D9PPA8
Locus tag DW289_RS25100 Protein ID WP_000405244.1
Coordinates 62005..62487 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID -
Locus tag DW289_RS25095 Protein ID WP_001346256.1
Coordinates 61712..62014 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DW289_RS25060 57412..58164 + 753 WP_089596776.1 conjugal transfer pilus acetylase TraX -
DW289_RS25065 58219..58779 + 561 WP_089596774.1 fertility inhibition protein FinO -
DW289_RS25070 58915..59127 + 213 WP_115431761.1 hypothetical protein -
DW289_RS25075 59322..59702 + 381 Protein_68 DUF2726 domain-containing protein -
- 59877..59938 - 62 NuclAT_1 - -
- 59877..59938 - 62 NuclAT_1 - -
- 59877..59938 - 62 NuclAT_1 - -
- 59877..59938 - 62 NuclAT_1 - -
DW289_RS25085 59982..60107 + 126 WP_115431769.1 type I toxin-antitoxin system Hok family toxin -
DW289_RS25090 60175..61351 + 1177 Protein_70 IS3 family transposase -
DW289_RS25095 61712..62014 + 303 WP_001346256.1 DUF1778 domain-containing protein Antitoxin
DW289_RS25100 62005..62487 + 483 WP_000405244.1 GNAT family N-acetyltransferase Toxin
DW289_RS25105 62797..63054 + 258 WP_000083825.1 replication regulatory protein RepA -
DW289_RS25690 63158..63356 + 199 Protein_74 protein CopA/IncA -
DW289_RS25120 63353..64210 + 858 WP_122990696.1 incFII family plasmid replication initiator RepA -
DW289_RS25130 65162..65413 + 252 WP_001139206.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
DW289_RS25135 65410..65697 + 288 WP_089584420.1 type II toxin-antitoxin system RelE/ParE family toxin -
DW289_RS25140 65987..66187 - 201 WP_089584419.1 alkaline phosphatase -
DW289_RS25145 66177..66382 + 206 Protein_79 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - paa / papH / papC / papD 1..82461 82461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-149)

Antitoxin

Vibrio phage ICP1, Orf50

(15-99)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17659.41 Da        Isoelectric Point: 7.1036

>T108930 WP_000405244.1 NZ_CP031323:62005-62487 [Escherichia coli]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLHGRAMKNQMLNASRTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T108930 NZ_CP031323:62005-62487 [Escherichia coli]
ATGGAAATAAATGTCACCGCACCGGCATTACTGACGGATGAGCATATACTTCAGCCATTTGATTGTGGGAATGAAGTGTT
AAGTAACTGGTTACATGGTCGCGCTATGAAAAACCAGATGCTCAATGCTTCCCGCACGTTCGTCATTTGCCTGGAGGGTA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACCGGTTCCGTCACTCATGCCGAACTTGGGCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTTCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACTCAGGGGCATGGTTTTGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGGTTGTCAATCTGGCTGATCAGGTTGGTATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGATGCCAGAGCGTTTTATGAGCGTTTTGGTTTTGTCCAGTCAGTTGTGGCACCAAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11602.19 Da        Isoelectric Point: 4.4569

>AT108930 WP_001346256.1 NZ_CP031323:61712-62014 [Escherichia coli]
MEVVMPTTASIRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDDECYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT108930 NZ_CP031323:61712-62014 [Escherichia coli]
ATGGAGGTTGTTATGCCTACTACTGCAAGCATCCGTAAAACACCGCGAGAAAATCAGATTAACATCCGGGCAACGGATGA
GGAACGAGCTGTTATTGATTATGCCGCTAGCCTGGTCAATAAGAACCGTACTGATTTCATCATTGAACTGGCTTACCAGG
AAGCTAAAAACATCATCCTGGATCAACGTTTATTTGTGCTGGATGATGAATGTTATGATTCATTTATTACGCAACTTGAA
GCTCCTGTGCAGAATGCTGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References