Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4231826..4232464 Replicon chromosome
Accession NZ_CP031321
Organism Escherichia coli strain ST2350 isolate Es_ST2350_SE1_NDM_03_2018

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag DW289_RS21590 Protein ID WP_000813794.1
Coordinates 4231826..4232002 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag DW289_RS21595 Protein ID WP_001270285.1
Coordinates 4232048..4232464 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DW289_RS21570 4227444..4228658 - 1215 WP_001326689.1 BenE family transporter YdcO -
DW289_RS21575 4228679..4229248 + 570 WP_115431705.1 DNA-binding transcriptional regulator SutR -
DW289_RS21580 4229321..4231282 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
DW289_RS21585 4231374..4231604 - 231 WP_000494244.1 YncJ family protein -
DW289_RS21590 4231826..4232002 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
DW289_RS21595 4232048..4232464 + 417 WP_001270285.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
DW289_RS21600 4232543..4233949 + 1407 WP_000760589.1 PLP-dependent aminotransferase family protein -
DW289_RS21605 4234194..4235339 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
DW289_RS21610 4235357..4236370 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
DW289_RS21615 4236371..4237312 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4226196..4227404 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(88-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T108916 WP_000813794.1 NZ_CP031321:4231826-4232002 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T108916 NZ_CP031321:4231826-4232002 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15247.61 Da        Isoelectric Point: 4.6115

>AT108916 WP_001270285.1 NZ_CP031321:4232048-4232464 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT108916 NZ_CP031321:4232048-4232464 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References