Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2480579..2481174 Replicon chromosome
Accession NZ_CP031321
Organism Escherichia coli strain ST2350 isolate Es_ST2350_SE1_NDM_03_2018

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag DW289_RS12635 Protein ID WP_000239579.1
Coordinates 2480824..2481174 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag DW289_RS12630 Protein ID WP_001223208.1
Coordinates 2480579..2480830 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DW289_RS12620 2476243..2480022 + 3780 WP_115431611.1 autotransporter assembly complex protein TamB -
DW289_RS12625 2480025..2480366 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
DW289_RS12630 2480579..2480830 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
DW289_RS12635 2480824..2481174 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
DW289_RS12640 2481254..2481784 - 531 WP_000055072.1 inorganic diphosphatase -
DW289_RS12645 2482094..2483050 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
DW289_RS12655 2483360..2484862 + 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
DW289_RS12660 2484876..2485898 + 1023 WP_001296689.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T108912 WP_000239579.1 NZ_CP031321:2480824-2481174 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T108912 NZ_CP031321:2480824-2481174 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT108912 WP_001223208.1 NZ_CP031321:2480579-2480830 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT108912 NZ_CP031321:2480579-2480830 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References