Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1042297..1042951 Replicon chromosome
Accession NZ_CP031321
Organism Escherichia coli strain ST2350 isolate Es_ST2350_SE1_NDM_03_2018

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag DW289_RS05170 Protein ID WP_000244772.1
Coordinates 1042297..1042704 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag DW289_RS05175 Protein ID WP_000354046.1
Coordinates 1042685..1042951 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DW289_RS05150 1038254..1039987 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
DW289_RS05155 1039993..1040703 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
DW289_RS05160 1040728..1041624 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
DW289_RS05165 1041736..1042257 + 522 WP_001055874.1 flavodoxin FldB -
DW289_RS05170 1042297..1042704 - 408 WP_000244772.1 protein YgfX Toxin
DW289_RS05175 1042685..1042951 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
DW289_RS05180 1043194..1044174 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
DW289_RS05185 1044251..1044910 - 660 WP_000250274.1 hemolysin III family protein -
DW289_RS05190 1045074..1045385 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
DW289_RS05195 1045430..1046863 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
DW289_RS05200 1046920..1047663 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T108906 WP_000244772.1 NZ_CP031321:c1042704-1042297 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T108906 NZ_CP031321:c1042704-1042297 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT108906 WP_000354046.1 NZ_CP031321:c1042951-1042685 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT108906 NZ_CP031321:c1042951-1042685 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References