Detailed information of TA system TA108886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1542214..1542761
• Replicon: chromosome
• Accession: NZ_CP031316
• Organism: Serratia marcescens strain N4-5

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A380ADA6
• Locus tag: Smn45_RS07420
• Protein ID: WP_025302144.1
• Coordinates: 1542453..1542761 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: Smn45_RS07415
• Protein ID: WP_025302143.1
• Coordinates: 1542214..1542450 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Smn45_RS07400	1538700..1540235	+	1536	WP_016928459.1	glutathione ABC transporter substrate-binding protein GsiB	-
Smn45_RS07405	1540288..1541208	+	921	WP_016928458.1	glutathione ABC transporter permease GsiC	-
Smn45_RS07410	1541218..1542126	+	909	WP_205407493.1	glutathione ABC transporter permease GsiD	-
Smn45_RS07415	1542214..1542450	+	237	WP_025302143.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
Smn45_RS07420	1542453..1542761	+	309	WP_025302144.1	CcdB family protein	Toxin
Smn45_RS07425	1542797..1543639	-	843	WP_015377214.1	S-formylglutathione hydrolase	-
Smn45_RS07430	1543654..1544778	-	1125	WP_016928454.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
Smn45_RS07435	1544809..1545729	-	921	WP_110146819.1	LysR family transcriptional regulator	-
Smn45_RS07440	1545835..1546992	+	1158	WP_033640883.1	YbfB/YjiJ family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11504.32 Da
• Isoelectric Point: 5.0196

>T108886 WP_025302144.1 NZ_CP031316:1542453-1542761 [Serratia marcescens]
MQFTVYDNTYPASAYPYLLDVQSDLIDVLSTRLMIPLYALDNVRVKISARLCPEIEVNGEKFLVMTHEMAAVRISQIGKA
VGNVNEHRNQIKAAIDFLIDGF

Nucleotide

• Length: 309 bp

>T108886 NZ_CP031316:1542453-1542761 [Serratia marcescens]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAACGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AGATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCTATCGATTTTCTGATTGATGGCTTTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8967.04 Da
• Isoelectric Point: 5.0807

>AT108886 WP_025302143.1 NZ_CP031316:1542214-1542450 [Serratia marcescens]
MANSNVTKKTVNVTIDRDLFQRAKSLGVNVSSVLTDALSVRVRDIEIQQWREQNRAALEELNRITEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT108886 NZ_CP031316:1542214-1542450 [Serratia marcescens]
ATGGCAAATAGCAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCCGTATTGACAGACGCATTGAGCGTCCGCGTCAGGGATATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGCGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTGA

Structures

Toxin

• AlphaFold DB: A0A380ADA6