Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1063329..1063950 Replicon chromosome
Accession NZ_CP031316
Organism Serratia marcescens strain N4-5

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag Smn45_RS05065 Protein ID WP_004940313.1
Coordinates 1063329..1063532 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag Smn45_RS05070 Protein ID WP_004940312.1
Coordinates 1063582..1063950 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Smn45_RS05035 1059030..1059368 + 339 WP_049299676.1 P-II family nitrogen regulator -
Smn45_RS05040 1059404..1060690 + 1287 WP_115435630.1 ammonium transporter AmtB -
Smn45_RS05045 1060781..1061644 - 864 WP_115435631.1 acyl-CoA thioesterase II -
Smn45_RS05050 1061875..1062366 + 492 WP_016928846.1 YbaY family lipoprotein -
Smn45_RS05055 1062431..1062745 - 315 WP_004940317.1 MGMT family protein -
Smn45_RS05065 1063329..1063532 - 204 WP_004940313.1 hemolysin expression modulator Hha Toxin
Smn45_RS05070 1063582..1063950 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
Smn45_RS05075 1064109..1064462 - 354 WP_016928845.1 hypothetical protein -
Smn45_RS05080 1064867..1065580 + 714 WP_115435632.1 ABC transporter ATP-binding protein -
Smn45_RS05085 1065577..1066434 + 858 WP_004940309.1 metal ABC transporter permease -
Smn45_RS05090 1066460..1067338 + 879 WP_016928843.1 metal ABC transporter substrate-binding protein -
Smn45_RS05095 1067446..1067586 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
Smn45_RS05100 1067599..1067853 - 255 WP_004940303.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T108884 WP_004940313.1 NZ_CP031316:c1063532-1063329 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T108884 NZ_CP031316:c1063532-1063329 [Serratia marcescens]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT108884 WP_004940312.1 NZ_CP031316:c1063950-1063582 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT108884 NZ_CP031316:c1063950-1063582 [Serratia marcescens]
ATGGATGAGTATTCGCCTAAGCGGCACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTATGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References