Detailed information of TA system TA108882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 6617..7118
• Replicon: plasmid pGD31-MCR
• Accession: NZ_CP031296
• Organism: Escherichia coli strain EC17GD31

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: DWB25_RS26280
• Protein ID: WP_000702247.1
• Coordinates: 6617..6877 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: DWB25_RS26285
• Protein ID: WP_001132407.1
• Coordinates: 6867..7118 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DWB25_RS26240	3123..3284	+	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-
DWB25_RS26245	3434..4030	+	597	WP_000125165.1	hypothetical protein	-
DWB25_RS28115	4048..4332	-	285	WP_001162371.1	hypothetical protein	-
DWB25_RS26255	4406..5071	-	666	WP_000532148.1	P-loop NTPase	-
DWB25_RS26265	5670..5993	+	324	WP_072834379.1	hypothetical protein	-
DWB25_RS26270	6035..6244	+	210	WP_000866036.1	hypothetical protein	-
DWB25_RS26275	6341..6565	+	225	WP_000814381.1	hypothetical protein	-
DWB25_RS26280	6617..6877	+	261	WP_000702247.1	hypothetical protein	Toxin
DWB25_RS26285	6867..7118	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
DWB25_RS26300	7668..7946	+	279	WP_000545938.1	hypothetical protein	-
DWB25_RS26305	8366..8632	+	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
DWB25_RS26310	8739..8921	-	183	WP_001481829.1	hypothetical protein	-
DWB25_RS26315	9479..9661	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
DWB25_RS26320	9686..10123	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
DWB25_RS26325	10254..10580	-	327	WP_000206697.1	hypothetical protein	-
DWB25_RS26330	10600..11307	-	708	WP_021503228.1	hypothetical protein	-
DWB25_RS26335	11309..12061	-	753	WP_000797966.1	conjugal transfer protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..60038	60038

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T108882 WP_000702247.1 NZ_CP031296:6617-6877 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T108882 NZ_CP031296:6617-6877 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT108882 WP_001132407.1 NZ_CP031296:6867-7118 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT108882 NZ_CP031296:6867-7118 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0