Detailed information of TA system TA108881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 230930..231465
• Replicon: plasmid pGD31-F1928
• Accession: NZ_CP031295
• Organism: Escherichia coli strain EC17GD31

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q3YTD6
• Locus tag: DWB25_RS26135
• Protein ID: WP_000222766.1
• Coordinates: 231178..231465 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: DWB25_RS26130
• Protein ID: WP_001132900.1
• Coordinates: 230930..231181 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DWB25_RS26075	225982..226725	+	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-
DWB25_RS26080	226780..227340	+	561	WP_000139310.1	fertility inhibition protein FinO	-
DWB25_RS26085	227475..227687	+	213	WP_001541896.1	ANR family transcriptional regulator	-
-	228029..228090	-	62	NuclAT_2	-	-
-	228029..228090	-	62	NuclAT_2	-	-
-	228029..228090	-	62	NuclAT_2	-	-
-	228029..228090	-	62	NuclAT_2	-	-
DWB25_RS26095	228077..228283	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	-
DWB25_RS26100	228551..228808	+	258	WP_001541895.1	replication regulatory protein RepA	-
DWB25_RS26110	229040..229114	+	75	WP_061357229.1	RepA leader peptide Tap	-
DWB25_RS26115	229107..229964	+	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
DWB25_RS26130	230930..231181	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DWB25_RS26135	231178..231465	+	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DWB25_RS26140	231540..231728	-	189	Protein_265	IS3 family transposase	-
DWB25_RS26145	231762..232262	-	501	Protein_266	Tn3 family transposase	-
DWB25_RS26150	232296..235301	-	3006	WP_001143760.1	Tn3-like element Tn3 family transposase	-
DWB25_RS26155	235465..236022	+	558	WP_001235713.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul3 / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) / tet(A) / aph(3')-Ia / sitABCD / blaTEM-1B	iucA / iucB / iucC / iucD / iutA	1..245305	245305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11147.23 Da
• Isoelectric Point: 10.5832

>T108881 WP_000222766.1 NZ_CP031295:231178-231465 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T108881 NZ_CP031295:231178-231465 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT108881 WP_001132900.1 NZ_CP031295:230930-231181 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT108881 NZ_CP031295:230930-231181 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A829CDZ2

Antitoxin

• AlphaFold DB: V0VIP2