Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4754828..4755444 Replicon chromosome
Accession NZ_CP031293
Organism Escherichia coli strain EC17GD31

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag DWB25_RS23000 Protein ID WP_001129490.1
Coordinates 4754828..4755202 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag DWB25_RS23005 Protein ID WP_001275523.1
Coordinates 4755202..4755444 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DWB25_RS22985 4752331..4753233 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
DWB25_RS22990 4753230..4753865 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
DWB25_RS22995 4753862..4754791 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
DWB25_RS23000 4754828..4755202 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
DWB25_RS23005 4755202..4755444 - 243 WP_001275523.1 ribbon-helix-helix domain-containing protein Antitoxin
DWB25_RS23010 4755664..4755882 - 219 WP_001251290.1 ribbon-helix-helix domain-containing protein -
DWB25_RS23015 4756556..4758370 - 1815 WP_000226324.1 hypothetical protein -
DWB25_RS23020 4758786..4759727 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
DWB25_RS23025 4759772..4760209 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T108862 WP_001129490.1 NZ_CP031293:c4755202-4754828 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T108862 NZ_CP031293:c4755202-4754828 [Escherichia coli]
ATGGCAAAAAGAACTGCGCTTTTTGATACCAATATCCTTATCGATTTGTTCAGCGGACGAATTGAGGCAAAACAGGCTCT
GGAGGCATGGCCCCTACAGAATGCGATTAGTCTGGTCACATGGATGGAGGTGCTGGTTGGCGCAAAAAAATATGATCAAG
AACATCGCACACGGATTGCAATGAGTGCGTTTAACGTTATTGGGATTTCGCAGGATATCGCGGAACGAAGCGTTGCTTTA
AGGCAGGAGTATAGAATGAAACTTCCAGATGCCATTATTCTGGCAACAGCACAGATTTATGGCTTTGAACTGGTAACGCG
AAATACGAGAGATTTTGCGGGAATAGCAGGTGTCGTCACACCCTATGAGCTGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT108862 WP_001275523.1 NZ_CP031293:c4755444-4755202 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT108862 NZ_CP031293:c4755444-4755202 [Escherichia coli]
ATGAGCGCTATGGCAGGTATGGATATGGGCAGAATTTTACTCGATTTATCCGATGAGGTGATTAAGCGACTTGATGATCT
CAAAGTGCAGCGCAATCTTCCTCGCGCAGAGCTATTACGAGAAGCTGTAGATCAATATCTGGAAAATCAATCGAAAACGA
CAATATCCAGTGCTTTTGGTATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References