Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 351888..352519 Replicon chromosome
Accession NZ_CP031293
Organism Escherichia coli strain EC17GD31

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag DWB25_RS01625 Protein ID WP_001260301.1
Coordinates 351888..352163 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag DWB25_RS01630 Protein ID WP_000593555.1
Coordinates 352160..352519 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DWB25_RS01610 346892..347959 + 1068 WP_000361497.1 HlyD family efflux transporter periplasmic adaptor subunit -
DWB25_RS01615 347956..350691 + 2736 WP_061092822.1 ribosome-associated ATPase/putative transporter RbbA -
DWB25_RS01620 350691..351815 + 1125 WP_001314210.1 ABC transporter permease -
DWB25_RS01625 351888..352163 + 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
DWB25_RS01630 352160..352519 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DWB25_RS01635 352639..353040 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
DWB25_RS01640 353045..353851 - 807 WP_061092823.1 nickel import ATP-binding protein NikE -
DWB25_RS01645 353848..354612 - 765 WP_001136211.1 nickel import ATP-binding protein NikD -
DWB25_RS01650 354612..355445 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
DWB25_RS01655 355442..356386 - 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 344904..353040 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T108838 WP_001260301.1 NZ_CP031293:351888-352163 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T108838 NZ_CP031293:351888-352163 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT108838 WP_000593555.1 NZ_CP031293:352160-352519 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT108838 NZ_CP031293:352160-352519 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References