Detailed information of TA system TA108836

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 70654..71452
• Replicon: chromosome
• Accession: NZ_CP031293
• Organism: Escherichia coli strain EC17GD31

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: DWB25_RS00300
• Protein ID: WP_061092894.1
• Coordinates: 70654..71031 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: DWB25_RS00305
• Protein ID: WP_061092893.1
• Coordinates: 71078..71452 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DWB25_RS00270	66017..66940	-	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-
DWB25_RS00275	67051..68235	-	1185	WP_001172864.1	sugar efflux transporter	-
DWB25_RS00280	68619..68765	-	147	WP_001328030.1	hypothetical protein	-
DWB25_RS00285	69033..69875	-	843	WP_061092922.1	DUF4942 domain-containing protein	-
DWB25_RS00290	69960..70157	-	198	WP_012908799.1	DUF957 domain-containing protein	-
DWB25_RS00295	70169..70657	-	489	WP_061092895.1	hypothetical protein	-
DWB25_RS00300	70654..71031	-	378	WP_061092894.1	TA system toxin CbtA family protein	Toxin
DWB25_RS00305	71078..71452	-	375	WP_061092893.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DWB25_RS00310	71615..71836	-	222	WP_061092892.1	DUF987 domain-containing protein	-
DWB25_RS00315	71899..72375	-	477	WP_001186188.1	RadC family protein	-
DWB25_RS00320	72390..72869	-	480	WP_000706978.1	antirestriction protein	-
DWB25_RS00325	73135..73953	-	819	WP_001175165.1	DUF945 domain-containing protein	-
DWB25_RS00330	74043..74276	-	234	WP_001278293.1	DUF905 family protein	-
DWB25_RS00335	74282..74959	-	678	WP_001097302.1	hypothetical protein	-
DWB25_RS00340	75107..75787	-	681	WP_001282921.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	59309..85015	25706

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.15 Da
• Isoelectric Point: 7.8045

>T108836 WP_061092894.1 NZ_CP031293:c71031-70654 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALIRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T108836 NZ_CP031293:c71031-70654 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGATACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAACATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13752.53 Da
• Isoelectric Point: 6.5924

>AT108836 WP_061092893.1 NZ_CP031293:c71452-71078 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNHLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT108836 NZ_CP031293:c71452-71078 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCACCTGCATTACCTTGCCGACCGCGCGGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA