Detailed information of TA system TA108835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2601599..2602250
• Replicon: chromosome
• Accession: NZ_CP031290
• Organism: Lactobacillus rhamnosus GG

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: DU507_RS13205
• Protein ID: WP_005686631.1
• Coordinates: 2601599..2601982 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: DU507_RS13210
• Protein ID: WP_005686632.1
• Coordinates: 2602002..2602250 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DU507_RS13190	2600635..2601162	-	528	WP_005686630.1	QueT transporter family protein	-
DU507_RS13195	2601200..2601397	-	198	WP_064536013.1	hypothetical protein	-
DU507_RS13205	2601599..2601982	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DU507_RS13210	2602002..2602250	-	249	WP_005686632.1	antitoxin	Antitoxin
DU507_RS13215	2602362..2603501	-	1140	WP_005686633.1	alanine racemase	-
DU507_RS13220	2603488..2603862	-	375	WP_005686634.1	holo-ACP synthase	-
DU507_RS13225	2604031..2605539	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
DU507_RS13230	2605813..2607201	-	1389	WP_015765066.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T108835 WP_005686631.1 NZ_CP031290:c2601982-2601599 [Lactobacillus rhamnosus GG]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T108835 NZ_CP031290:c2601982-2601599 [Lactobacillus rhamnosus GG]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAATAACGTTGGCAATCACTATAGTCCCACCGTCATTGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACTCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
AGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT108835 WP_005686632.1 NZ_CP031290:c2602250-2602002 [Lactobacillus rhamnosus GG]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT108835 NZ_CP031290:c2602250-2602002 [Lactobacillus rhamnosus GG]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGTGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAGTTGATTT
CGGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19