Detailed information of TA system TA108814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 795565..796089
• Replicon: chromosome
• Accession: NZ_CP031274
• Organism: Staphylococcus chromogenes strain 17A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DWB97_RS03925
• Protein ID: WP_103159488.1
• Coordinates: 795736..796089 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A3A0K2V6
• Locus tag: DWB97_RS03920
• Protein ID: WP_037577177.1
• Coordinates: 795565..795735 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DWB97_RS03895	791413..791886	+	474	WP_145400619.1	PH domain-containing protein	-
DWB97_RS03900	791879..793357	+	1479	WP_145400620.1	PH domain-containing protein	-
DWB97_RS03905	793354..793869	+	516	WP_165885554.1	PH domain-containing protein	-
DWB97_RS03910	793866..794216	+	351	WP_145400622.1	holo-ACP synthase	-
DWB97_RS03915	794332..795480	+	1149	WP_037577179.1	alanine racemase	-
DWB97_RS03920	795565..795735	+	171	WP_037577177.1	hypothetical protein	Antitoxin
DWB97_RS03925	795736..796089	+	354	WP_103159488.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DWB97_RS03930	796153..797157	+	1005	WP_145400623.1	PP2C family protein-serine/threonine phosphatase	-
DWB97_RS03935	797238..797564	+	327	WP_145400624.1	anti-sigma factor antagonist	-
DWB97_RS03940	797567..798046	+	480	WP_037577169.1	anti-sigma B factor RsbW	-
DWB97_RS03945	798021..798791	+	771	WP_037577167.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13064.34 Da
• Isoelectric Point: 10.5127

>T108814 WP_103159488.1 NZ_CP031274:795736-796089 [Staphylococcus chromogenes]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIDKKKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSDKKMKEVNAALGISLGLHMNQHK

Nucleotide

• Length: 354 bp

>T108814 NZ_CP031274:795736-796089 [Staphylococcus chromogenes]
ATGAAGCGTGGCGATGTTTATTTAGCGGATTTATCACCAGTACAAGGGTCAGAGCAAGGGGGAGTCAGACCTGTCGTTAT
TATTCAAAATGATACTGGTAATAAATACAGTCCGACCGTGATTGTTGCAGCGATTACAGGTCGCATCAATAAAGCGAAAA
TTCCAACGCATGTCGAAATTGATAAGAAAAAATATAAACTCGACAAAGATTCTGTTATTTTGTTAGAACAAATTAGAACA
GTCGATAAAAAGAGGCTTAAAGAAAAGCTCACCTTTCTTTCTGATAAAAAGATGAAAGAAGTCAATGCGGCACTTGGAAT
TAGCTTAGGATTGCACATGAATCAACACAAATAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6328.01 Da
• Isoelectric Point: 4.2313

>AT108814 WP_037577177.1 NZ_CP031274:795565-795735 [Staphylococcus chromogenes]
MSAFNQTRVKSLEQSLKEGYVQMADLNLSLATEAYFVECEACDCNESHLLSNHKDD

Nucleotide

• Length: 171 bp

>AT108814 NZ_CP031274:795565-795735 [Staphylococcus chromogenes]
ATGTCAGCATTCAATCAAACAAGAGTGAAAAGTTTAGAGCAATCTTTGAAAGAGGGGTATGTTCAGATGGCAGATTTAAA
TCTCTCCCTAGCAACAGAAGCATACTTTGTCGAATGTGAAGCATGTGATTGCAACGAATCACACTTACTTTCTAACCACA
AGGATGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A3A0K2V6