Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF-doc
Location 24846..25429 Replicon plasmid unnamed1
Accession NZ_CP031272
Organism Staphylococcus caprae strain 26D

Toxin (Protein)


Gene name pemK Uniprot ID C5QR44
Locus tag DWB96_RS13170 Protein ID WP_002442047.1
Coordinates 25091..25429 (+) Length 113 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID C5QR43
Locus tag DWB96_RS13165 Protein ID WP_002442045.1
Coordinates 24846..25094 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DWB96_RS13130 20095..20442 - 348 WP_002441960.1 metalloregulator ArsR/SmtB family transcription factor -
DWB96_RS13135 20461..21078 - 618 WP_104681722.1 CadD family cadmium resistance transporter -
DWB96_RS13140 21276..21812 + 537 WP_145376084.1 hypothetical protein -
DWB96_RS13150 22692..23339 - 648 Protein_26 protein rep -
DWB96_RS13155 23399..24073 - 675 Protein_27 IS6 family transposase -
DWB96_RS13160 24132..24497 + 366 Protein_28 hypothetical protein -
DWB96_RS13165 24846..25094 + 249 WP_002442045.1 hypothetical protein Antitoxin
DWB96_RS13170 25091..25429 + 339 WP_002442047.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DWB96_RS13175 25667..26341 + 675 Protein_31 IS6 family transposase -
DWB96_RS13185 26552..26908 + 357 WP_145376078.1 hypothetical protein -
DWB96_RS13190 27626..27844 + 219 WP_000153199.1 hypothetical protein -
DWB96_RS13195 28028..28363 - 336 WP_145376080.1 SdpI family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaZ - 1..28583 28583
- inside IScluster/Tn - - 23930..25810 1880


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-106)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 13134.25 Da        Isoelectric Point: 9.6404

>T108813 WP_002442047.1 NZ_CP031272:25091-25429 [Staphylococcus caprae]
MSIKQFDIYYIDLDPTRGREKQKIRPCLIVNNNMTIKGTDFVWVLPITNREKRYPLDIEVKTKKGLVTGVIDTLQIRALD
LNEREHNYKDELQDNLKNVVLQAIKTYLKPSS

Download         Length: 339 bp

>T108813 NZ_CP031272:25091-25429 [Staphylococcus caprae]
ATGAGTATCAAACAGTTTGATATATACTATATTGACCTAGACCCCACTCGAGGTCGTGAAAAACAAAAAATTAGACCATG
CTTAATCGTGAATAACAATATGACCATTAAAGGAACAGATTTTGTTTGGGTATTACCAATTACAAATCGTGAAAAACGTT
ATCCATTAGATATCGAAGTGAAAACTAAAAAAGGATTAGTCACAGGTGTTATAGACACGCTTCAGATACGCGCATTAGAC
CTTAATGAACGTGAACATAACTATAAAGATGAATTACAAGACAACTTAAAAAACGTCGTCTTACAAGCAATAAAGACGTA
TTTAAAACCATCATCATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9210.33 Da        Isoelectric Point: 4.5463

>AT108813 WP_002442045.1 NZ_CP031272:24846-25094 [Staphylococcus caprae]
MIKTSERIFKSGNSKALSLSKQTIQLADFEVGDKVEVSEINGGLFIKKKEESIEDEITNFFKNGGKYTEPEFDFGESVGR
EI

Download         Length: 249 bp

>AT108813 NZ_CP031272:24846-25094 [Staphylococcus caprae]
ATGATTAAAACATCTGAACGCATTTTTAAAAGTGGAAACAGCAAAGCACTCAGTTTAAGCAAACAAACAATCCAGTTAGC
TGATTTTGAGGTGGGAGATAAGGTTGAAGTATCTGAAATCAATGGTGGTTTATTTATCAAAAAGAAAGAAGAATCGATTG
AAGATGAAATCACAAATTTTTTCAAAAATGGCGGTAAATACACCGAGCCAGAATTTGATTTTGGAGAGAGTGTAGGTCGT
GAAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A269XML6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J6P0H1

References