Detailed information of TA system TA108787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 5558..6183
• Replicon: chromosome
• Accession: NZ_CP031263
• Organism: Micromonospora aurantiaca strain 110B(2018)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DVH21_RS00035
• Protein ID: WP_114918531.1
• Coordinates: 5764..6183 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3M9KLX8
• Locus tag: DVH21_RS00030
• Protein ID: WP_013475596.1
• Coordinates: 5558..5767 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVH21_RS00010	1285..2730	+	1446	WP_047893734.1	alpha-amylase family protein	-
DVH21_RS00015	2804..3694	+	891	WP_030271669.1	L,D-transpeptidase	-
DVH21_RS00020	3735..4478	-	744	WP_013287866.1	3-oxoacyl-ACP reductase FabG	-
DVH21_RS00025	4618..5469	+	852	WP_091331026.1	alpha/beta fold hydrolase	-
DVH21_RS00030	5558..5767	+	210	WP_013475596.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DVH21_RS00035	5764..6183	+	420	WP_114918531.1	PIN domain nuclease	Toxin
DVH21_RS00040	6366..6620	-	255	WP_114918532.1	hypothetical protein	-
DVH21_RS00045	6617..6931	-	315	WP_114918533.1	DivIVA domain-containing protein	-
DVH21_RS00050	7218..8000	-	783	WP_114918534.1	transposase family protein	-
DVH21_RS00055	8154..8957	+	804	WP_114918535.1	helix-turn-helix domain-containing protein	-
DVH21_RS00060	8971..9177	+	207	WP_091331023.1	DUF397 domain-containing protein	-
DVH21_RS00065	9266..9688	+	423	WP_091331022.1	hypothetical protein	-
DVH21_RS00070	9780..10721	+	942	WP_161801647.1	hypothetical protein	-
DVH21_RS00075	10752..11168	+	417	WP_141722605.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	7218..8000	782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-119)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15406.70 Da
• Isoelectric Point: 6.6416

>T108787 WP_114918531.1 NZ_CP031263:5764-6183 [Micromonospora aurantiaca]
MTRERYLLDKSALARWPKPAVAPVLDELSDRGLLAVCGAIEIEVVHSTRSAKDAKRARWLLRGFDWLAMPDDIWDRAIDI
QVQALHKGNHRALSMADLLIAATAERHGVTVLHYDGDFDLITAITGQPTTWVVPAGKAD

Nucleotide

• Length: 420 bp

>T108787 NZ_CP031263:5764-6183 [Micromonospora aurantiaca]
ATGACGCGGGAGCGCTACCTCCTCGACAAGTCGGCGCTGGCCCGGTGGCCGAAGCCCGCCGTGGCGCCGGTCCTGGACGA
GTTGTCCGATCGCGGTCTGCTGGCGGTCTGCGGCGCCATCGAGATCGAGGTCGTACACAGCACCCGCTCGGCCAAGGACG
CGAAGCGCGCTCGATGGCTGCTCCGCGGTTTCGACTGGCTCGCCATGCCCGACGACATCTGGGATCGTGCGATCGACATC
CAGGTGCAGGCGCTGCACAAGGGCAACCACCGCGCGCTCTCGATGGCCGATCTGCTGATCGCCGCGACGGCCGAACGGCA
TGGCGTGACCGTTCTGCACTACGACGGTGACTTCGACCTGATCACCGCGATCACCGGCCAGCCGACGACGTGGGTCGTCC
CGGCCGGGAAGGCCGACTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7395.28 Da
• Isoelectric Point: 4.3719

>AT108787 WP_013475596.1 NZ_CP031263:5558-5767 [Micromonospora aurantiaca]
MSRTILDVDDDLLAEAAEILGTGTKKATVNAALQEVVNREKRREFADWLKSGGLPDLTDPGQASKPEAA

Nucleotide

• Length: 210 bp

>AT108787 NZ_CP031263:5558-5767 [Micromonospora aurantiaca]
ATGTCCCGCACCATTCTCGATGTCGACGACGACCTCCTGGCCGAGGCCGCCGAGATCCTCGGCACGGGCACCAAGAAGGC
CACTGTCAACGCCGCGCTGCAGGAAGTGGTCAACCGGGAGAAGCGCCGGGAGTTCGCCGACTGGCTCAAGAGCGGCGGCC
TGCCGGACCTCACCGACCCGGGGCAGGCGTCGAAGCCCGAAGCGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A3M9KLX8