Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 84358..84883 Replicon plasmid pL22-5
Accession NZ_CP031262
Organism Klebsiella quasipneumoniae strain L22

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag DV026_RS28550 Protein ID WP_013023785.1
Coordinates 84578..84883 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag DV026_RS28545 Protein ID WP_001568025.1
Coordinates 84358..84576 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DV026_RS28510 79514..80293 + 780 WP_013023780.1 hypothetical protein -
DV026_RS28515 80500..82092 + 1593 WP_015344964.1 hypothetical protein -
DV026_RS28520 82126..83253 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
DV026_RS28525 83250..83540 + 291 WP_013023783.1 hypothetical protein -
DV026_RS28530 83530..83727 - 198 WP_017899886.1 hypothetical protein -
DV026_RS28545 84358..84576 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DV026_RS28550 84578..84883 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
DV026_RS28555 85052..85447 + 396 WP_017899885.1 hypothetical protein -
DV026_RS28560 85474..85788 + 315 WP_053389906.1 hypothetical protein -
DV026_RS28565 85799..86815 + 1017 WP_017899884.1 hypothetical protein -
DV026_RS28570 87013..87807 + 795 WP_004197635.1 site-specific integrase -
DV026_RS28575 88263..88442 - 180 WP_006788217.1 hypothetical protein -
DV026_RS28580 88562..89188 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 / blaTEM-1B - 1..95314 95314


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T108786 WP_013023785.1 NZ_CP031262:84578-84883 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T108786 NZ_CP031262:84578-84883 [Klebsiella quasipneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT108786 WP_001568025.1 NZ_CP031262:84358-84576 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT108786 NZ_CP031262:84358-84576 [Klebsiella quasipneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References