Detailed information of TA system TA108785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 184321..185057
• Replicon: plasmid pL22-1
• Accession: NZ_CP031258
• Organism: Klebsiella quasipneumoniae strain L22

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: DV026_RS26680
• Protein ID: WP_117985291.1
• Coordinates: 184575..185057 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A7D3T1D0
• Locus tag: DV026_RS26675
• Protein ID: WP_004213599.1
• Coordinates: 184321..184587 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV026_RS26650	179553..180110	+	558	WP_004213590.1	recombinase family protein	-
DV026_RS26655	180113..183082	+	2970	WP_004213592.1	Tn3 family transposase	-
DV026_RS26660	183124..183618	+	495	WP_004213594.1	hypothetical protein	-
DV026_RS26665	183679..183882	+	204	WP_004213596.1	hemolysin expression modulator Hha	-
DV026_RS26670	183896..184126	+	231	WP_004213598.1	hypothetical protein	-
DV026_RS26675	184321..184587	+	267	WP_004213599.1	DUF1778 domain-containing protein	Antitoxin
DV026_RS26680	184575..185057	+	483	WP_117985291.1	GNAT family N-acetyltransferase	Toxin
DV026_RS26685	185478..186705	+	1228	Protein_197	IS3 family transposase	-
DV026_RS26690	186701..187096	-	396	Protein_198	DDE-type integrase/transposase/recombinase	-
DV026_RS26700	187271..188293	-	1023	WP_004214536.1	porphobilinogen synthase	-
DV026_RS26705	188357..189703	-	1347	WP_048333011.1	dihydroorotase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / rmpA / iutA / iucD / iucC / iucB / iucA	1..212635	212635
	-	inside	IScluster/Tn	-	-	179553..186705	7152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T108785 WP_117985291.1 NZ_CP031258:184575-185057 [Klebsiella quasipneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENLGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T108785 NZ_CP031258:184575-185057 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACCTTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5141

>AT108785 WP_004213599.1 NZ_CP031258:184321-184587 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT108785 NZ_CP031258:184321-184587 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7D3T1D0