Detailed information of TA system TA108784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 118940..119610
• Replicon: plasmid pL22-1
• Accession: NZ_CP031258
• Organism: Klebsiella quasipneumoniae strain L22

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: DV026_RS26310
• Protein ID: WP_004213072.1
• Coordinates: 119167..119610 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: DV026_RS26305
• Protein ID: WP_004213073.1
• Coordinates: 118940..119170 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV026_RS26280	115163..116062	-	900	WP_004225022.1	nucleotide-binding protein	-
DV026_RS26285	116052..116342	-	291	WP_004213078.1	hypothetical protein	-
DV026_RS26290	116694..116900	+	207	WP_004213077.1	hypothetical protein	-
DV026_RS26295	116890..117183	-	294	WP_004213076.1	hypothetical protein	-
DV026_RS26300	117199..118332	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
DV026_RS26305	118940..119170	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DV026_RS26310	119167..119610	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
DV026_RS26315	119624..120040	-	417	Protein_130	restriction endonuclease	-
DV026_RS26320	120044..120337	-	294	Protein_131	transposase	-
DV026_RS29300	120754..121391	+	638	Protein_132	response regulator	-
DV026_RS26340	121906..122312	-	407	Protein_133	GAF domain-containing protein	-
DV026_RS26350	122403..123323	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
DV026_RS26355	123372..123863	-	492	WP_011251327.1	DM13 domain-containing protein	-
DV026_RS26360	123926..124201	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / rmpA / iutA / iucD / iucC / iucB / iucA	1..212635	212635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T108784 WP_004213072.1 NZ_CP031258:119167-119610 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T108784 NZ_CP031258:119167-119610 [Klebsiella quasipneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT108784 WP_004213073.1 NZ_CP031258:118940-119170 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT108784 NZ_CP031258:118940-119170 [Klebsiella quasipneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620