Detailed information of TA system TA108765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4812790..4813366
• Replicon: chromosome
• Accession: NZ_CP031256
• Organism: Escherichia coli strain M16807

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A061KXE4
• Locus tag: DV447_RS24720
• Protein ID: WP_001295743.1
• Coordinates: 4813079..4813366 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A066STF6
• Locus tag: DV447_RS24715
• Protein ID: WP_000063148.1
• Coordinates: 4812790..4813092 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV447_RS24700	4809386..4811536	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
DV447_RS24705	4811586..4811789	+	204	WP_000467859.1	YbdD/YjiX family protein	-
DV447_RS24710	4811800..4812756	+	957	WP_001295745.1	GTPase	-
DV447_RS24715	4812790..4813092	-	303	WP_000063148.1	BrnA antitoxin family protein	Antitoxin
DV447_RS24720	4813079..4813366	-	288	WP_001295743.1	BrnT family toxin	Toxin
DV447_RS24725	4813563..4814729	+	1167	WP_000800831.1	restriction endonuclease	-
DV447_RS24730	4814793..4816412	+	1620	WP_001029745.1	type I restriction-modification system subunit M	-
DV447_RS24735	4816402..4817706	+	1305	WP_000535012.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11156.62 Da
• Isoelectric Point: 7.4697

>T108765 WP_001295743.1 NZ_CP031256:c4813366-4813079 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T108765 NZ_CP031256:c4813366-4813079 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11592.12 Da
• Isoelectric Point: 9.4646

>AT108765 WP_000063148.1 NZ_CP031256:c4813092-4812790 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT108765 NZ_CP031256:c4813092-4812790 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A061KXE4

Antitoxin

• AlphaFold DB: A0A066STF6