Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4213518..4214136 Replicon chromosome
Accession NZ_CP031256
Organism Escherichia coli strain M16807

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DV447_RS21670 Protein ID WP_001291435.1
Coordinates 4213918..4214136 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DV447_RS21665 Protein ID WP_000344800.1
Coordinates 4213518..4213892 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DV447_RS21655 4208608..4209801 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DV447_RS21660 4209824..4212973 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
DV447_RS21665 4213518..4213892 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DV447_RS21670 4213918..4214136 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DV447_RS21675 4214309..4214860 + 552 WP_000102539.1 maltose O-acetyltransferase -
DV447_RS21680 4214976..4215446 + 471 WP_001304825.1 YlaC family protein -
DV447_RS21685 4215610..4217160 + 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DV447_RS21690 4217202..4217555 - 354 WP_000878140.1 DUF1428 domain-containing protein -
DV447_RS21700 4217934..4218245 + 312 WP_000409908.1 MGMT family protein -
DV447_RS21705 4218276..4218848 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T108760 WP_001291435.1 NZ_CP031256:4213918-4214136 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T108760 NZ_CP031256:4213918-4214136 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT108760 WP_000344800.1 NZ_CP031256:4213518-4213892 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT108760 NZ_CP031256:4213518-4213892 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References