Detailed information of TA system TA108759

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3568143..3568977
• Replicon: chromosome
• Accession: NZ_CP031256
• Organism: Escherichia coli strain M16807

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: DV447_RS18355
• Protein ID: WP_000854690.1
• Coordinates: 3568143..3568520 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: DV447_RS18360
• Protein ID: WP_001546171.1
• Coordinates: 3568609..3568977 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV447_RS18320	3563734..3564288	-	555	WP_001001909.1	molecular chaperone YcdY	-
DV447_RS18325	3564312..3565049	-	738	WP_000283664.1	zinc-binding phosphatase	-
DV447_RS18330	3565104..3566042	-	939	WP_000351287.1	glyoxylate/hydroxypyruvate reductase GhrA	-
DV447_RS18340	3566529..3567356	-	828	WP_001304477.1	DUF4942 domain-containing protein	-
DV447_RS18345	3567441..3567638	-	198	WP_000772027.1	DUF957 domain-containing protein	-
DV447_RS18350	3567658..3568146	-	489	WP_001546173.1	hypothetical protein	-
DV447_RS18355	3568143..3568520	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
DV447_RS18360	3568609..3568977	-	369	WP_001546171.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DV447_RS18365	3569027..3569671	-	645	WP_000094915.1	hypothetical protein	-
DV447_RS18370	3569690..3569911	-	222	WP_000692329.1	DUF987 domain-containing protein	-
DV447_RS18375	3569974..3570450	-	477	WP_001537421.1	RadC family protein	-
DV447_RS18380	3570466..3570939	-	474	WP_000855076.1	antirestriction protein	-
DV447_RS18385	3571202..3572023	-	822	WP_001234710.1	DUF945 domain-containing protein	-
DV447_RS26800	3572104..3572315	+	212	Protein_3362	hypothetical protein	-
DV447_RS18395	3572434..3572889	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T108759 WP_000854690.1 NZ_CP031256:c3568520-3568143 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T108759 NZ_CP031256:c3568520-3568143 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13745.37 Da
• Isoelectric Point: 4.6220

>AT108759 WP_001546171.1 NZ_CP031256:c3568977-3568609 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT108759 NZ_CP031256:c3568977-3568609 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: S1PLF5