108758

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3473783..3474254 Replicon chromosome
Accession NZ_CP031256
Organism Escherichia coli strain M16807

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag DV447_RS17790 Protein ID WP_001303511.1
Coordinates 3473976..3474254 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag DV447_RS17785 Protein ID WP_001302048.1
Coordinates 3473783..3473974 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DV447_RS17740 3469032..3469250 - 219 WP_000209148.1 DUF4014 family protein -
DV447_RS17745 3469283..3469495 - 213 WP_000072553.1 hypothetical protein -
DV447_RS17750 3469601..3470023 - 423 Protein_3245 DUF977 family protein -
DV447_RS17755 3470039..3470809 - 771 WP_000450998.1 DUF1627 domain-containing protein -
DV447_RS17760 3470831..3471577 - 747 WP_000788950.1 ATP-binding protein -
DV447_RS17765 3471584..3472546 - 963 WP_000095673.1 helix-turn-helix domain-containing protein -
DV447_RS17770 3472569..3472994 - 426 WP_000693943.1 Rha family transcriptional regulator -
DV447_RS17775 3472991..3473293 - 303 WP_001556930.1 transcriptional regulator -
DV447_RS17780 3473391..3473762 + 372 WP_001169687.1 hypothetical protein -
DV447_RS17785 3473783..3473974 + 192 WP_001302048.1 hypothetical protein Antitoxin
DV447_RS17790 3473976..3474254 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DV447_RS17800 3474546..3474698 + 153 WP_000380313.1 DUF1391 family protein -
DV447_RS17805 3474710..3475048 + 339 WP_000394541.1 hypothetical protein -
DV447_RS17810 3475037..3475303 - 267 WP_012601410.1 hypothetical protein -
DV447_RS17825 3475807..3475995 + 189 WP_000449175.1 cell division inhibitor -
DV447_RS17830 3475992..3476180 + 189 WP_000199475.1 DUF1482 family protein -
DV447_RS17835 3476273..3478711 + 2439 WP_000102132.1 exonuclease -
DV447_RS17840 3478773..3479042 + 270 WP_000003742.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3371172..3487297 116125


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T108758 WP_001303511.1 NZ_CP031256:3473976-3474254 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T108758 NZ_CP031256:3473976-3474254 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT108758 WP_001302048.1 NZ_CP031256:3473783-3473974 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT108758 NZ_CP031256:3473783-3473974 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
AlphaFold DB A0A829CTP0

References