Detailed information of TA system TA108741

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 520955..521746
• Replicon: chromosome
• Accession: NZ_CP031256
• Organism: Escherichia coli strain M16807

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: DV447_RS02540
• Protein ID: WP_000691791.1
• Coordinates: 520955..521380 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: DV447_RS02545
• Protein ID: WP_000939437.1
• Coordinates: 521411..521746 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV447_RS02505	516621..517118	-	498	WP_000509808.1	hypothetical protein	-
DV447_RS02515	517800..518186	-	387	WP_001270938.1	DUF2441 domain-containing protein	-
DV447_RS02525	518809..519540	-	732	WP_001216639.1	TIGR02646 family protein	-
DV447_RS02530	519537..519758	-	222	WP_125112077.1	hypothetical protein	-
DV447_RS02535	519743..520934	-	1192	Protein_450	AAA family ATPase	-
DV447_RS02540	520955..521380	-	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
DV447_RS02545	521411..521746	-	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DV447_RS02550	521746..522219	-	474	WP_001292742.1	DNA repair protein RadC	-
DV447_RS02555	522249..523063	-	815	Protein_454	DUF932 domain-containing protein	-
DV447_RS02560	523299..524252	-	954	WP_000290406.1	hypothetical protein	-
DV447_RS02565	524845..525459	+	615	WP_000772910.1	inovirus Gp2 family protein	-
DV447_RS02570	525577..525795	+	219	WP_000070772.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	518809..543993	25184

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T108741 WP_000691791.1 NZ_CP031256:c521380-520955 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T108741 NZ_CP031256:c521380-520955 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCTCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT108741 WP_000939437.1 NZ_CP031256:c521746-521411 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT108741 NZ_CP031256:c521746-521411 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6