Detailed information of TA system TA108730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/YoeB-YefM
• Location: 563957..564465
• Replicon: chromosome
• Accession: NZ_CP031251
• Organism: Neisseria subflava strain M18660

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: DV114_RS02755
• Protein ID: WP_079453728.1
• Coordinates: 563957..564220 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: DV114_RS02760
• Protein ID: WP_101756423.1
• Coordinates: 564217..564465 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV114_RS11215	559909..560172	+	264	WP_143485327.1	zeta toxin family protein	-
DV114_RS11220	560254..560493	+	240	WP_143485328.1	hypothetical protein	-
DV114_RS02730	560645..561226	+	582	WP_101756425.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
DV114_RS02735	561228..561428	+	201	Protein_516	Y-family DNA polymerase	-
DV114_RS11225	561435..561815	-	381	WP_162817313.1	hypothetical protein	-
DV114_RS02740	561841..562581	-	741	WP_101756420.1	hypothetical protein	-
DV114_RS11230	562592..562741	-	150	WP_159068741.1	hypothetical protein	-
DV114_RS02745	562772..562951	-	180	WP_101756421.1	hypothetical protein	-
DV114_RS02750	563527..563910	+	384	WP_101756422.1	hypothetical protein	-
DV114_RS02755	563957..564220	-	264	WP_079453728.1	Txe/YoeB family addiction module toxin	Toxin
DV114_RS02760	564217..564465	-	249	WP_101756423.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
DV114_RS02765	564977..565738	-	762	Protein_524	hypothetical protein	-
DV114_RS02770	565823..566479	+	657	WP_101804488.1	IS1595 family transposase	-
DV114_RS02775	566509..566991	+	483	WP_101755618.1	site-specific integrase	-
DV114_RS02810	567867..568427	-	561	WP_003679552.1	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	-
DV114_RS02815	568545..569027	-	483	Protein_528	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	543737..566991	23254
	-	flank	IS/Tn	-	-	565823..566479	656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-75)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10475.94 Da
• Isoelectric Point: 10.0980

>T108730 WP_079453728.1 NZ_CP031251:c564220-563957 [Neisseria subflava]
VRKHRFTADAAEDLAYWKKHDARKVERIKLLLEDIKANHPKGIGKPEPLRHHKSGLWSRRIDREHRLVYSVDEDTVTVYA
CRYHYER

Nucleotide

• Length: 264 bp

>T108730 NZ_CP031251:c564220-563957 [Neisseria subflava]
GTGAGGAAGCACCGTTTTACCGCTGATGCTGCCGAAGATTTGGCATATTGGAAAAAGCATGATGCTAGGAAGGTTGAACG
GATTAAGTTGCTTTTGGAAGATATTAAAGCCAACCATCCGAAGGGAATCGGTAAACCCGAACCACTTCGTCACCATAAAT
CGGGCTTATGGTCGCGAAGGATTGATCGGGAACACCGTTTGGTTTATTCGGTAGATGAAGATACGGTAACTGTATATGCC
TGCCGATACCATTACGAACGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9245.38 Da
• Isoelectric Point: 4.0737

>AT108730 WP_101756423.1 NZ_CP031251:c564465-564217 [Neisseria subflava]
MDYVISYTAAREDLAQVMTKVCEDQVVTHITRRNGGNCVLMSEEEYSSIMETLYLFGNPINAARLEHSLAQAEQGEFVEV
DL

Nucleotide

• Length: 249 bp

>AT108730 NZ_CP031251:c564465-564217 [Neisseria subflava]
ATGGACTATGTAATCAGCTACACCGCGGCCCGTGAAGATTTGGCGCAAGTTATGACTAAAGTTTGTGAAGACCAAGTAGT
AACACATATTACGCGCCGGAACGGAGGGAACTGTGTCTTGATGTCTGAGGAAGAGTACAGCTCTATTATGGAGACACTTT
ACCTGTTCGGAAACCCTATTAATGCTGCACGTTTGGAACACTCGCTGGCGCAAGCAGAACAGGGGGAATTTGTAGAGGTT
GATCTGTGA