Detailed information of TA system TA108727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 350438..350916
• Replicon: chromosome
• Accession: NZ_CP031250
• Organism: Haemophilus influenzae strain M21384

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: -
• Locus tag: DV389_RS01780
• Protein ID: WP_114892945.1
• Coordinates: 350438..350716 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: A0A0K9KX05
• Locus tag: DV389_RS01785
• Protein ID: WP_012055040.1
• Coordinates: 350725..350916 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV389_RS01760	345628..347298	-	1671	WP_114892941.1	fructose-specific PTS transporter subunit EIIC	-
DV389_RS01765	347300..348241	-	942	WP_114892942.1	1-phosphofructokinase	-
DV389_RS01770	348243..349742	-	1500	WP_114892943.1	fused PTS fructose transporter subunit IIA/HPr protein	-
DV389_RS01775	349812..350342	-	531	WP_162816713.1	hypothetical protein	-
DV389_RS01780	350438..350716	-	279	WP_114892945.1	virulence factor	Toxin
DV389_RS01785	350725..350916	-	192	WP_012055040.1	DUF5397 family protein	Antitoxin
DV389_RS01790	350987..352285	-	1299	WP_074040071.1	hemolysin family protein	-
DV389_RS01795	352336..352860	-	525	WP_114892946.1	DUF1523 family protein	-
DV389_RS01800	352887..353669	-	783	WP_114892947.1	YchF/TatD family DNA exonuclease	-
DV389_RS01805	353708..354688	-	981	WP_114892948.1	DNA polymerase III subunit delta'	-
DV389_RS01810	354685..355344	-	660	WP_114892949.1	dTMP kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (1-63)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10635.00 Da
• Isoelectric Point: 5.7408

>T108727 WP_114892945.1 NZ_CP031250:c350716-350438 [Haemophilus influenzae]
MYAIAFDLVVKDTQDYHPKGVQQAYTDIGAVLAKFGFVRTQGSLYTNTNEDMANLFQAMNSLKQLTWISQSVRDIRAFRI
EQWSDFTDFIRS

Nucleotide

• Length: 279 bp

>T108727 NZ_CP031250:c350716-350438 [Haemophilus influenzae]
ATGTACGCGATTGCTTTTGATTTAGTTGTAAAAGACACTCAAGATTATCATCCAAAAGGTGTTCAGCAGGCTTATACAGA
CATTGGTGCGGTTTTAGCAAAATTTGGTTTTGTTAGAACACAAGGAAGTTTATATACCAATACAAATGAAGATATGGCAA
ACCTTTTTCAGGCAATGAATTCGCTAAAACAGTTGACGTGGATTTCTCAGTCGGTACGAGATATTCGTGCTTTTCGTATT
GAGCAATGGTCTGACTTCACTGACTTTATACGAAGTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7018.97 Da
• Isoelectric Point: 3.9930

>AT108727 WP_012055040.1 NZ_CP031250:c350916-350725 [Haemophilus influenzae]
MELRQQIPTGCIKQFGQFGVPYVVGEVAEFLPDGDALVNITLLQSGEKDIYRLSHLLEDPEAE

Nucleotide

• Length: 192 bp

>AT108727 NZ_CP031250:c350916-350725 [Haemophilus influenzae]
ATGGAACTAAGACAACAAATTCCAACAGGCTGTATTAAGCAATTTGGGCAATTTGGCGTGCCTTATGTGGTTGGCGAGGT
TGCGGAATTTTTGCCAGATGGCGATGCGTTGGTAAATATCACATTGTTACAATCTGGAGAAAAAGATATTTATCGCTTAT
CTCACTTACTTGAAGATCCCGAAGCTGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0K9KX05