Detailed information of TA system TA108704

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1475433..1476070
• Replicon: chromosome
• Accession: NZ_CP031242
• Organism: Haemophilus influenzae strain M21460

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: DV390_RS07430
• Protein ID: WP_005649049.1
• Coordinates: 1475433..1475837 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: DV390_RS07435
• Protein ID: WP_005649046.1
• Coordinates: 1475834..1476070 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV390_RS07400	1470993..1471559	+	567	WP_005544066.1	elongation factor P	-
DV390_RS07410	1471910..1472497	-	588	WP_005654063.1	primosomal replication protein	-
DV390_RS07415	1472530..1473882	-	1353	WP_005694344.1	Na+/H+ antiporter family protein	-
DV390_RS07420	1474196..1474885	-	690	WP_011271988.1	ribonuclease T	-
DV390_RS07425	1474959..1475366	-	408	WP_005688980.1	lactoylglutathione lyase	-
DV390_RS07430	1475433..1475837	-	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
DV390_RS07435	1475834..1476070	-	237	WP_005649046.1	antitoxin	Antitoxin
DV390_RS07440	1476163..1476888	-	726	WP_011271987.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
DV390_RS07445	1476941..1477459	-	519	WP_005649040.1	isoprenylcysteine carboxyl methyltransferase family protein	-
DV390_RS07450	1477678..1479444	+	1767	WP_042594297.1	aspartate--tRNA ligase	-
DV390_RS07455	1479466..1479939	+	474	WP_011271985.1	dihydroneopterin triphosphate diphosphatase	-
DV390_RS07460	1479951..1480691	+	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T108704 WP_005649049.1 NZ_CP031242:c1475837-1475433 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T108704 NZ_CP031242:c1475837-1475433 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT108704 WP_005649046.1 NZ_CP031242:c1476070-1475834 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT108704 NZ_CP031242:c1476070-1475834 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3