Detailed information of TA system TA108677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 99029..99504
• Replicon: chromosome
• Accession: NZ_CP031237
• Organism: Haemophilus influenzae strain M12125

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: -
• Locus tag: DV365_RS00510
• Protein ID: WP_005693712.1
• Coordinates: 99229..99504 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: A0A0M1GIA6
• Locus tag: DV365_RS00505
• Protein ID: WP_005649350.1
• Coordinates: 99029..99220 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV365_RS00480	94608..95240	+	633	WP_032828392.1	dTMP kinase	-
DV365_RS00485	95237..96220	+	984	WP_010868989.1	DNA polymerase III subunit delta'	-
DV365_RS00490	96275..97057	+	783	WP_005693710.1	YchF/TatD family DNA exonuclease	-
DV365_RS00495	97084..97608	+	525	WP_005652096.1	DUF1523 family protein	-
DV365_RS00500	97659..98957	+	1299	WP_005693711.1	hemolysin family protein	-
DV365_RS00505	99029..99220	+	192	WP_005649350.1	DUF5397 domain-containing protein	Antitoxin
DV365_RS00510	99229..99504	+	276	WP_005693712.1	endoribonuclease VapD	Toxin
DV365_RS00515	99597..100127	+	531	WP_005686915.1	hypothetical protein	-
DV365_RS00520	100199..101698	+	1500	WP_112101779.1	fused PTS fructose transporter subunit IIA/HPr protein	-
DV365_RS00525	101700..102641	+	942	WP_005693714.1	1-phosphofructokinase	-
DV365_RS00530	102643..104313	+	1671	WP_005693715.1	fructose-specific PTS transporter subunit EIIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (6-85)

Antitoxin

• Protein of unknown function DUF5397 (1-63)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10543.06 Da
• Isoelectric Point: 7.0053

>T108677 WP_005693712.1 NZ_CP031237:99229-99504 [Haemophilus influenzae]
MYAIAFLVVKDTQDYHPKGVQQAYTDIGAVLAKFGFVRTQGSLYINMNEDMANLFQAMNALKQLAWISQSVRDIRAFRIE
QWSDFTDFIRN

Nucleotide

• Length: 276 bp

>T108677 NZ_CP031237:99229-99504 [Haemophilus influenzae]
ATGTACGCGATTGCTTTTTTAGTTGTAAAAGACACTCAAGATTATCATCCAAAGGGCGTTCAACAGGCTTATACAGACAT
TGGCGCAGTCTTAGCAAAATTTGGTTTTGTTAGAACACAAGGAAGTTTATATATCAACATGAATGAAGATATGGCGAATC
TCTTTCAGGCAATGAATGCACTAAAACAGTTGGCGTGGATTTCTCAGTCGGTACGAGATATTCGTGCTTTTCGTATTGAG
CAATGGTCTGATTTTACTGATTTTATACGAAATTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7047.02 Da
• Isoelectric Point: 3.9930

>AT108677 WP_005649350.1 NZ_CP031237:99029-99220 [Haemophilus influenzae]
MELRQQIPTGCIKQFGQFGVPYVVGEVAEFLPDGDVLVNITLLQSGEKDIYRLSHLLEDPEAE

Nucleotide

• Length: 192 bp

>AT108677 NZ_CP031237:99029-99220 [Haemophilus influenzae]
ATGGAACTAAGACAACAAATTCCAACAGGCTGTATTAAGCAATTCGGGCAATTTGGCGTGCCTTATGTGGTTGGCGAGGT
TGCGGAATTTTTGCCAGATGGTGATGTGTTGGTGAATATCACATTGTTACAATCTGGTGAAAAAGACATTTATCGCTTAT
CTCACCTGCTTGAAGATCCCGAAGCTGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0M1GIA6