Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 11225..11747 Replicon plasmid pEsST410_NW_4
Accession NZ_CP031234
Organism Escherichia coli strain Es_ST410_NW1_NDM_09_2017

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag DVH14_RS24695 Protein ID WP_000220561.1
Coordinates 11225..11506 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag DVH14_RS24700 Protein ID WP_000121743.1
Coordinates 11496..11747 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DVH14_RS24655 7208..7753 - 546 WP_001426313.1 hypothetical protein -
DVH14_RS25770 7748..7822 - 75 Protein_10 DNA distortion polypeptide 3 -
DVH14_RS24660 8079..8351 - 273 WP_000167280.1 hypothetical protein -
DVH14_RS25645 8368..8514 - 147 WP_001567307.1 hypothetical protein -
DVH14_RS24670 8806..9135 - 330 WP_000866654.1 hypothetical protein -
DVH14_RS24675 9229..9471 - 243 WP_000008708.1 hypothetical protein -
DVH14_RS24680 9461..9673 - 213 WP_001446884.1 hypothetical protein -
DVH14_RS24685 9739..10287 - 549 WP_001178641.1 hypothetical protein -
DVH14_RS24690 10361..10876 - 516 WP_001025387.1 J domain-containing protein -
DVH14_RS24695 11225..11506 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DVH14_RS24700 11496..11747 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
DVH14_RS24705 12812..13681 + 870 WP_032152971.1 replication initiation protein -
DVH14_RS24710 13686..14520 + 835 Protein_21 RepB family plasmid replication initiator protein -
DVH14_RS24715 14524..14967 + 444 WP_053220570.1 hypothetical protein -
DVH14_RS24720 15038..15460 + 423 WP_000202949.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkF / mrkD / mrkC / mrkB / mrkA 1..46994 46994


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T108670 WP_000220561.1 NZ_CP031234:c11506-11225 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T108670 NZ_CP031234:c11506-11225 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT108670 WP_000121743.1 NZ_CP031234:c11747-11496 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT108670 NZ_CP031234:c11747-11496 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References